Pretreatment Method for DNA Barcoding to Analyze Gut Contents of Rotifers

Pretreatment Method for DNA Barcoding to Analyze Gut Contents of Rotifers

We designed an experiment to analyze the gut content of Rotifera based on DNA barcoding and tested it on <i>Asplanchna</i> sp. in order to ensure that the DNA extracted from the rotifer species is from the food sources within the gut. We selected ethanol fixation (60%) to minimize the in...

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Bibliographic Details
Main Authors:	Hye-Ji Oh, Paul Henning Krogh, Hyun-Gi Jeong, Gea-Jae Joo, Ihn-Sil Kwak, Sun-Jin Hwang, Jeong-Soo Gim, Kwang-Hyeon Chang, Hyunbin Jo
Format:	Article
Language:	English
Published:	MDPI AG 2020-02-01
Series:	Applied Sciences
Subjects:	gut content of rotifera eliminate the extracellular dna commercial bleach pretreatment
Online Access:	https://www.mdpi.com/2076-3417/10/3/1064

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