HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes

HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes

Primary endothelial cells (ECs), especially human umbilical vein endothelial cells (HUVECs), are broadly used in vascular biology. Gene editing of primary endothelial cells is known to be challenging, due to the low DNA transfection efficiency and the limited proliferation capacity of ECs. We report...

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Bibliographic Details
Main Authors: Camilla Blunk Brandt, Sofie Vestergaard Fonager, János Haskó, Rikke Bek Helmig, Søren Degn, Lars Bolund, Niels Jessen, Lin Lin, Yonglun Luo
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Biomolecules
Subjects:
endothelial cells
human umbilical vein endothelial cells
HIF1A
hypoxia inducible factor 1 alpha
CRISPR-Cas
gene editing
Online Access:https://www.mdpi.com/2218-273X/13/1/23

Internet

https://www.mdpi.com/2218-273X/13/1/23

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