Dual guide RNA-mediated concurrent C&G-to-T&A and A&T-to-G&C conversions using CRISPR base editors

Dual guide RNA-mediated concurrent C&G-to-T&A and A&T-to-G&C conversions using CRISPR base editors

Base editing tools enable precise genome modifications, disease modeling, and promising gene therapy. However, many human genetic diseases are elicited by multi-nucleotide variants (MNVs) with heterogeneous substitutions at the same genomic locus. Based on the adenine and cytosine base editors, dual...

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Bibliographic Details
Main Authors: Yuting Zhao, Min Li, Jie Liu, Xiaowen Xue, Jingli Zhong, Jianxiang Lin, Bo Ye, Jun Chen, Yunbo Qiao
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
Dual guide RNAs (gRNAs)
Multi-nucleotide variant (MNV)
C&G-to-T&A and A&T-to-G&C conversions
TP53
SpRY
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037022006109

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http://www.sciencedirect.com/science/article/pii/S2001037022006109

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