Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies
Abstract A candidate multigenic SARS-CoV-2 vaccine based on an MVA vector expressing both viral N and S proteins (MVA-S + N) was immunogenic, and induced T-cell responses and binding antibodies to both antigens but in the absence of detectable neutralizing antibodies. Intranasal immunization with th...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-11-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-021-00405-5 |
| _version_ | 1827613503886721024 |
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| author | Chaojie Zhong Hongjie Xia Awadalkareem Adam Binbin Wang Renee L. Hajnik Yuejin Liang Grace H. Rafael Jing Zou Xiaofang Wang Jiaren Sun Lynn Soong Alan D. T. Barrett Scott C. Weaver Pei-Yong Shi Tian Wang Haitao Hu |
| author_facet | Chaojie Zhong Hongjie Xia Awadalkareem Adam Binbin Wang Renee L. Hajnik Yuejin Liang Grace H. Rafael Jing Zou Xiaofang Wang Jiaren Sun Lynn Soong Alan D. T. Barrett Scott C. Weaver Pei-Yong Shi Tian Wang Haitao Hu |
| author_sort | Chaojie Zhong |
| collection | DOAJ |
| description | Abstract A candidate multigenic SARS-CoV-2 vaccine based on an MVA vector expressing both viral N and S proteins (MVA-S + N) was immunogenic, and induced T-cell responses and binding antibodies to both antigens but in the absence of detectable neutralizing antibodies. Intranasal immunization with the vaccine diminished viral loads and lung inflammation in mice after SARS-CoV-2 challenge, which correlated with the T-cell response induced by the vaccine in the lung, indicating that T-cell immunity is also likely critical for protection against SARS-CoV-2 infection in addition to neutralizing antibodies. |
| first_indexed | 2024-03-09T08:40:41Z |
| format | Article |
| id | doaj.art-378daaf3c6264d6f95e72af6146211c7 |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-09T08:40:41Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-378daaf3c6264d6f95e72af6146211c72023-12-02T16:49:23ZengNature Portfolionpj Vaccines2059-01052021-11-01611710.1038/s41541-021-00405-5Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodiesChaojie Zhong0Hongjie Xia1Awadalkareem Adam2Binbin Wang3Renee L. Hajnik4Yuejin Liang5Grace H. Rafael6Jing Zou7Xiaofang Wang8Jiaren Sun9Lynn Soong10Alan D. T. Barrett11Scott C. Weaver12Pei-Yong Shi13Tian Wang14Haitao Hu15Department of Microbiology and Immunology, University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Pathology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Biochemistry and Molecular Biology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchAbstract A candidate multigenic SARS-CoV-2 vaccine based on an MVA vector expressing both viral N and S proteins (MVA-S + N) was immunogenic, and induced T-cell responses and binding antibodies to both antigens but in the absence of detectable neutralizing antibodies. Intranasal immunization with the vaccine diminished viral loads and lung inflammation in mice after SARS-CoV-2 challenge, which correlated with the T-cell response induced by the vaccine in the lung, indicating that T-cell immunity is also likely critical for protection against SARS-CoV-2 infection in addition to neutralizing antibodies.https://doi.org/10.1038/s41541-021-00405-5 |
| spellingShingle | Chaojie Zhong Hongjie Xia Awadalkareem Adam Binbin Wang Renee L. Hajnik Yuejin Liang Grace H. Rafael Jing Zou Xiaofang Wang Jiaren Sun Lynn Soong Alan D. T. Barrett Scott C. Weaver Pei-Yong Shi Tian Wang Haitao Hu Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies npj Vaccines |
| title | Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies |
| title_full | Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies |
| title_fullStr | Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies |
| title_full_unstemmed | Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies |
| title_short | Mucosal vaccination induces protection against SARS-CoV-2 in the absence of detectable neutralizing antibodies |
| title_sort | mucosal vaccination induces protection against sars cov 2 in the absence of detectable neutralizing antibodies |
| url | https://doi.org/10.1038/s41541-021-00405-5 |
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