Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment
Stroke is predominantly a senescent disease, yet most preclinical studies investigate treatment in young animals. We recently demonstrated that short-duration hypothermia-treatment completely prevented the dramatic intracranial pressure (ICP) rise seen post-stroke in young rats. Here, our aim was to...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-05-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnagi.2016.00124/full |
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| author | Lucy Anne Murtha Daniel Joseph Beard Julia eBourke Debbie ePepperall Damian Donald McLeod Neil James Spratt |
| author_facet | Lucy Anne Murtha Daniel Joseph Beard Julia eBourke Debbie ePepperall Damian Donald McLeod Neil James Spratt |
| author_sort | Lucy Anne Murtha |
| collection | DOAJ |
| description | Stroke is predominantly a senescent disease, yet most preclinical studies investigate treatment in young animals. We recently demonstrated that short-duration hypothermia-treatment completely prevented the dramatic intracranial pressure (ICP) rise seen post-stroke in young rats. Here, our aim was to investigate whether a similar ICP rise occurs in aged rats and to determine whether short-duration hypothermia is an effective treatment in aged animals. Experimental Middle Cerebral Artery occlusion (MCAo - 3 hour occlusion) was performed on male Wistar rats aged 19-20 months. At one hour after stroke-onset, rats were randomized to 2.5 hours hypothermia-treatment (32.5 °C) or normothermia (37 °C). ICP was monitored at baseline, for 3.5 hours post-occlusion, and at 24 hours post-stroke. Infarct and edema volumes were calculated from histology. Baseline pre-stroke ICP was 11.2 ± 3.3 mmHg across all animals. Twenty-four hours post-stroke, ICP was significantly higher in normothermic animals compared to hypothermia-treated animals (27.4 ± 18.2 mmHg vs. 8.0 ± 5.0 mmHg, p = 0.03). Infarct and edema volumes were not significantly different between groups. These data demonstrate ICP may also increase 24 hours post-stroke in aged rats, and that short-duration hypothermia treatment has a profound and sustained preventative effect. These findings may have important implications for the use of hypothermia in clinical trials of aged stroke patients. |
| first_indexed | 2024-12-11T22:52:28Z |
| format | Article |
| id | doaj.art-379d7449ae2843ffaed263d99a884416 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-12-11T22:52:28Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-379d7449ae2843ffaed263d99a8844162022-12-22T00:47:24ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652016-05-01810.3389/fnagi.2016.00124198392Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia TreatmentLucy Anne Murtha0Daniel Joseph Beard1Julia eBourke2Debbie ePepperall3Damian Donald McLeod4Neil James Spratt5The University of NewcastleThe University of NewcastleThe University of NewcastleThe University of NewcastleThe University of NewcastleThe University of NewcastleStroke is predominantly a senescent disease, yet most preclinical studies investigate treatment in young animals. We recently demonstrated that short-duration hypothermia-treatment completely prevented the dramatic intracranial pressure (ICP) rise seen post-stroke in young rats. Here, our aim was to investigate whether a similar ICP rise occurs in aged rats and to determine whether short-duration hypothermia is an effective treatment in aged animals. Experimental Middle Cerebral Artery occlusion (MCAo - 3 hour occlusion) was performed on male Wistar rats aged 19-20 months. At one hour after stroke-onset, rats were randomized to 2.5 hours hypothermia-treatment (32.5 °C) or normothermia (37 °C). ICP was monitored at baseline, for 3.5 hours post-occlusion, and at 24 hours post-stroke. Infarct and edema volumes were calculated from histology. Baseline pre-stroke ICP was 11.2 ± 3.3 mmHg across all animals. Twenty-four hours post-stroke, ICP was significantly higher in normothermic animals compared to hypothermia-treated animals (27.4 ± 18.2 mmHg vs. 8.0 ± 5.0 mmHg, p = 0.03). Infarct and edema volumes were not significantly different between groups. These data demonstrate ICP may also increase 24 hours post-stroke in aged rats, and that short-duration hypothermia treatment has a profound and sustained preventative effect. These findings may have important implications for the use of hypothermia in clinical trials of aged stroke patients.http://journal.frontiersin.org/Journal/10.3389/fnagi.2016.00124/fullIntracranial Pressurecerebral edemamiddle cerebral artery occlusionischaemic strokeAged ratsTherapuetic Hypothermia |
| spellingShingle | Lucy Anne Murtha Daniel Joseph Beard Julia eBourke Debbie ePepperall Damian Donald McLeod Neil James Spratt Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment Frontiers in Aging Neuroscience Intracranial Pressure cerebral edema middle cerebral artery occlusion ischaemic stroke Aged rats Therapuetic Hypothermia |
| title | Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment |
| title_full | Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment |
| title_fullStr | Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment |
| title_full_unstemmed | Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment |
| title_short | Intracranial Pressure Elevation 24 Hours after Ischemic Stroke in Aged Rats is Prevented by Early, Short Hypothermia Treatment |
| title_sort | intracranial pressure elevation 24 hours after ischemic stroke in aged rats is prevented by early short hypothermia treatment |
| topic | Intracranial Pressure cerebral edema middle cerebral artery occlusion ischaemic stroke Aged rats Therapuetic Hypothermia |
| url | http://journal.frontiersin.org/Journal/10.3389/fnagi.2016.00124/full |
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