Bone to pick: the importance of evaluating reference genes for RT-qPCR quantification of gene expression in craniosynostosis and bone-related tissues and cells

<p>Abstract</p> <p>Background</p> <p>RT-qPCR is a common tool for quantification of gene expression, but its accuracy is dependent on the choice and stability (steady state expression levels) of the reference gene/s used for normalization. To date, in the bone field, th...

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Bibliographic Details
Main Authors: Yang Xianxian, Hatfield Jodie T, Hinze Susan J, Mu Xiongzheng, Anderson Peter J, Powell Barry C
Format: Article
Language:English
Published: BMC 2012-05-01
Series:BMC Research Notes
Subjects:
Online Access:http://www.biomedcentral.com/1756-0500/5/222