Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific degradation of tagged endogenous proteins in vivo. Most...

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Bibliographic Details
Main Authors: Lewis Macdonald, Gillian C Taylor, Jennifer Margaret Brisbane, Ersi Christodoulou, Lucy Scott, Alex von Kriegsheim, Janet Rossant, Bin Gu, Andrew J Wood
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2022-06-01
Series:eLife
Subjects:
degron
genetic tools
condensin
Online Access:https://elifesciences.org/articles/77987

Internet

https://elifesciences.org/articles/77987

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