Screening and validation of reference genes for qRT-PCR of bovine skeletal muscle-derived satellite cells

Abstract The accuracy of sixteen commonly used internal reference genes was assessed in skeletal muscle-derived satellite cells of Qinchuan cattle at different stages of proliferation and induction of differentiation to determine the most suitable ones. Quantitative real-time PCR and three commonly...

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Bibliographic Details
Main Authors: Guo-Hua Wang, Cheng-Cheng Liang, Bing-Zhi Li, Xin-Ze Du, Wen-Zhen Zhang, Gong Cheng, Lin-Sen Zan
Format: Article
Language:English
Published: Nature Portfolio 2022-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-09476-3