Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease
Objective: This study aimed to investigate the effect of interleukin (IL)-21 and B cell lymphoma protein-6 on germinal center follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells and its relationship with the clinical features of inflammatory bowel disease (IBD).Methods: The expre...
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| Format: | Article |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2020.587445/full |
| _version_ | 1818456330604642304 |
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| author | Youguang Yang Xiaodan Lv Lingling Zhan Lan Chen Hui Jin Xinping Tang Qingqing Shi Qiyuan Zou Jiqiao Xiang WeiWei Zhang Zhaojing Zeng Haixing Jiang Xiaoping Lv |
| author_facet | Youguang Yang Xiaodan Lv Lingling Zhan Lan Chen Hui Jin Xinping Tang Qingqing Shi Qiyuan Zou Jiqiao Xiang WeiWei Zhang Zhaojing Zeng Haixing Jiang Xiaoping Lv |
| author_sort | Youguang Yang |
| collection | DOAJ |
| description | Objective: This study aimed to investigate the effect of interleukin (IL)-21 and B cell lymphoma protein-6 on germinal center follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells and its relationship with the clinical features of inflammatory bowel disease (IBD).Methods: The expression of peripheral blood cytokines IL-21 and Bcl-6 mRNA was detected by reverse transcription–polymerase chain reaction. The distribution characteristics of Tfh and Tfr cells were detected using the triple immunofluorescence staining analysis.Results: The expression of IL-21 and Bcl-6 mRNA was upregulated in the ulcerative colitis (UC) and Crohn disease (CD) groups compared with that in the control group. Triple immunofluorescence staining showed that the number of Tfh cells in the intestinal germinal center obviously increased in the UC and CD groups compared with that in the control group, whereas the number of Tfr cells reduced.Conclusion: This study suggested that the Tfr and Tfh cells might be involved in the regulation of IBD. Bcl-6 and IL-21 can regulate the Tfh/Tfr ratio in the intestinal germinal center, promoting the occurrence and development of IBD. |
| first_indexed | 2024-12-14T22:24:57Z |
| format | Article |
| id | doaj.art-37ecd438d6b7468cb5d3a8b37092aae7 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-14T22:24:57Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-37ecd438d6b7468cb5d3a8b37092aae72022-12-21T22:45:24ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-01-011110.3389/fphar.2020.587445587445Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel DiseaseYouguang Yang0Xiaodan Lv1Lingling Zhan2Lan Chen3Hui Jin4Xinping Tang5Qingqing Shi6Qiyuan Zou7Jiqiao Xiang8WeiWei Zhang9Zhaojing Zeng10Haixing Jiang11Xiaoping Lv12Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Clinical Experimental Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Clinical Experimental Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaObjective: This study aimed to investigate the effect of interleukin (IL)-21 and B cell lymphoma protein-6 on germinal center follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells and its relationship with the clinical features of inflammatory bowel disease (IBD).Methods: The expression of peripheral blood cytokines IL-21 and Bcl-6 mRNA was detected by reverse transcription–polymerase chain reaction. The distribution characteristics of Tfh and Tfr cells were detected using the triple immunofluorescence staining analysis.Results: The expression of IL-21 and Bcl-6 mRNA was upregulated in the ulcerative colitis (UC) and Crohn disease (CD) groups compared with that in the control group. Triple immunofluorescence staining showed that the number of Tfh cells in the intestinal germinal center obviously increased in the UC and CD groups compared with that in the control group, whereas the number of Tfr cells reduced.Conclusion: This study suggested that the Tfr and Tfh cells might be involved in the regulation of IBD. Bcl-6 and IL-21 can regulate the Tfh/Tfr ratio in the intestinal germinal center, promoting the occurrence and development of IBD.https://www.frontiersin.org/articles/10.3389/fphar.2020.587445/fullBcl-6follicular helper T cellsfollicular regulatory T cellsgerminal centerIL-21inflammatory bowel disease |
| spellingShingle | Youguang Yang Xiaodan Lv Lingling Zhan Lan Chen Hui Jin Xinping Tang Qingqing Shi Qiyuan Zou Jiqiao Xiang WeiWei Zhang Zhaojing Zeng Haixing Jiang Xiaoping Lv Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease Frontiers in Pharmacology Bcl-6 follicular helper T cells follicular regulatory T cells germinal center IL-21 inflammatory bowel disease |
| title | Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease |
| title_full | Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease |
| title_fullStr | Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease |
| title_full_unstemmed | Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease |
| title_short | Case Report: IL-21 and Bcl-6 Regulate the Proliferation and Secretion of Tfh and Tfr Cells in the Intestinal Germinal Center of Patients With Inflammatory Bowel Disease |
| title_sort | case report il 21 and bcl 6 regulate the proliferation and secretion of tfh and tfr cells in the intestinal germinal center of patients with inflammatory bowel disease |
| topic | Bcl-6 follicular helper T cells follicular regulatory T cells germinal center IL-21 inflammatory bowel disease |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2020.587445/full |
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