A CRISPR/dCas9 toolkit for functional analysis of maize genes

A CRISPR/dCas9 toolkit for functional analysis of maize genes

Abstract Background The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. The RNA-guided nuclease can be used to not only generate precise genomic mutations, but also to manipulate gene expression when present...

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Bibliographic Details
Main Authors: Irene N. Gentzel, Chan Ho Park, Maria Bellizzi, Guiqing Xiao, Kiran R. Gadhave, Colin Murphree, Qin Yang, Jonathan LaMantia, Margaret G. Redinbaugh, Peter Balint-Kurti, Tim L. Sit, Guo-Liang Wang
Format: Article
Language:English
Published: BMC 2020-10-01
Series:Plant Methods
Subjects:
Maize
Protoplasts
CRISPR/Cas9
Transcription activation
Transcription suppression
Online Access:http://link.springer.com/article/10.1186/s13007-020-00675-5

Internet

http://link.springer.com/article/10.1186/s13007-020-00675-5

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