ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples

Summary: In this protocol, we describe the use of ChipCytometry to combine RNA in situ hybridization and antibody staining for multiplexed tissue imaging of human formalin-fixed and paraffin-embedded tissue samples. The advantages of ChipCytometry are long-term storage for re-interrogation and advan...

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Bibliographic Details
Main Authors: Sebastian Jarosch, Jan Köhlen, Sabrina Wagner, Elvira D’Ippolito, Dirk H. Busch
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002544