ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples
Summary: In this protocol, we describe the use of ChipCytometry to combine RNA in situ hybridization and antibody staining for multiplexed tissue imaging of human formalin-fixed and paraffin-embedded tissue samples. The advantages of ChipCytometry are long-term storage for re-interrogation and advan...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002544 |
| _version_ | 1818205690940882944 |
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| author | Sebastian Jarosch Jan Köhlen Sabrina Wagner Elvira D’Ippolito Dirk H. Busch |
| author_facet | Sebastian Jarosch Jan Köhlen Sabrina Wagner Elvira D’Ippolito Dirk H. Busch |
| author_sort | Sebastian Jarosch |
| collection | DOAJ |
| description | Summary: In this protocol, we describe the use of ChipCytometry to combine RNA in situ hybridization and antibody staining for multiplexed tissue imaging of human formalin-fixed and paraffin-embedded tissue samples. The advantages of ChipCytometry are long-term storage for re-interrogation and advanced image quality by high dynamic range imaging of staining and background. A titrated pretreatment of tissue samples bypasses challenges because of the retrieval of antigens on coverslips and achieves an optimal staining quality at the minimal expense of tissue integrity.For complete details on the use and execution of this protocol, please refer to Jarosch et al. (2021). |
| first_indexed | 2024-12-12T04:01:09Z |
| format | Article |
| id | doaj.art-37f4e557819443adb0ef626a7b822f58 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T04:01:09Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-37f4e557819443adb0ef626a7b822f582022-12-22T00:39:02ZengElsevierSTAR Protocols2666-16672022-06-0132101374ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samplesSebastian Jarosch0Jan Köhlen1Sabrina Wagner2Elvira D’Ippolito3Dirk H. Busch4Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich (TUM), 81675 Munich, Bavaria, Germany; Corresponding authorInstitute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich (TUM), 81675 Munich, Bavaria, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich (TUM), 81675 Munich, Bavaria, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich (TUM), 81675 Munich, Bavaria, GermanyInstitute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich (TUM), 81675 Munich, Bavaria, Germany; Corresponding authorSummary: In this protocol, we describe the use of ChipCytometry to combine RNA in situ hybridization and antibody staining for multiplexed tissue imaging of human formalin-fixed and paraffin-embedded tissue samples. The advantages of ChipCytometry are long-term storage for re-interrogation and advanced image quality by high dynamic range imaging of staining and background. A titrated pretreatment of tissue samples bypasses challenges because of the retrieval of antigens on coverslips and achieves an optimal staining quality at the minimal expense of tissue integrity.For complete details on the use and execution of this protocol, please refer to Jarosch et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722002544Single CellMicroscopyMolecular BiologyAntibodyIn Situ Hybridization |
| spellingShingle | Sebastian Jarosch Jan Köhlen Sabrina Wagner Elvira D’Ippolito Dirk H. Busch ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples STAR Protocols Single Cell Microscopy Molecular Biology Antibody In Situ Hybridization |
| title | ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples |
| title_full | ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples |
| title_fullStr | ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples |
| title_full_unstemmed | ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples |
| title_short | ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples |
| title_sort | chipcytometry for multiplexed detection of protein and mrna markers on human ffpe tissue samples |
| topic | Single Cell Microscopy Molecular Biology Antibody In Situ Hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722002544 |
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