CRISPR/Cas9-mediated knockout of intracellular molecule SHP-1 enhances tumor-killing ability of CD133-targeted CAR T cells in vitro

CRISPR/Cas9-mediated knockout of intracellular molecule SHP-1 enhances tumor-killing ability of CD133-targeted CAR T cells in vitro

Abstract CAR T cell therapy has been successfully used in the treatment of hematological malignancies, and the strategy that deletion of inhibitory receptor on the CAR T cell surface, such as PD-1, greatly enhance the antitumor effects. Here, we describe a one-step electroporation for the co-transfe...

Full description

Bibliographic Details
Main Authors: Ming Liu, Linlin Zhang, Mingtian Zhong, Yihao Long, Wenhui Yang, Ting Liu, Xingxu Huang, Xiaodong Ma
Format: Article
Language:English
Published: BMC 2023-10-01
Series:Experimental Hematology & Oncology
Subjects:
SHP-1
CD133
CAR
CRISPR/Cas9
PiggyBac Transposase
Online Access:https://doi.org/10.1186/s40164-023-00450-x

Internet

https://doi.org/10.1186/s40164-023-00450-x

Similar Items