GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000
The nonprotein amino acid γ-aminobutyric acid (GABA) is the most abundant amino acid in the tomato (Solanum lycopersicum) leaf apoplast and is synthesized by Arabidopsis thaliana in response to infection by the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (hereafter called DC3000). High...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2016-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-16-0172-R |
| _version_ | 1811278101343109120 |
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| author | S. L. McCraw D. H. Park R. Jones M. A. Bentley A. Rico R. G. Ratcliffe N. J. Kruger A. Collmer G. M. Preston |
| author_facet | S. L. McCraw D. H. Park R. Jones M. A. Bentley A. Rico R. G. Ratcliffe N. J. Kruger A. Collmer G. M. Preston |
| author_sort | S. L. McCraw |
| collection | DOAJ |
| description | The nonprotein amino acid γ-aminobutyric acid (GABA) is the most abundant amino acid in the tomato (Solanum lycopersicum) leaf apoplast and is synthesized by Arabidopsis thaliana in response to infection by the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (hereafter called DC3000). High levels of exogenous GABA have previously been shown to repress the expression of the type III secretion system (T3SS) in DC3000, resulting in reduced elicitation of the hypersensitive response (HR) in the nonhost plant tobacco (Nicotiana tabacum). This study demonstrates that the GABA permease GabP provides the primary mechanism for GABA uptake by DC3000 and that the gabP deletion mutant ΔgabP is insensitive to GABA-mediated repression of T3SS expression. ΔgabP displayed an enhanced ability to elicit the HR in young tobacco leaves and in tobacco plants engineered to produce increased levels of GABA, which supports the hypothesis that GABA uptake via GabP acts to regulate T3SS expression in planta. The observation that P. syringae can be rendered insensitive to GABA through loss of gabP but that gabP is retained by this bacterium suggests that GabP is important for DC3000 in a natural setting, either for nutrition or as a mechanism for regulating gene expression.[Graphic: see text] Copyright © 2016 The Author(s). This is an open access article distributed under the CC BY Attribution 4.0 International license. |
| first_indexed | 2024-04-13T00:28:37Z |
| format | Article |
| id | doaj.art-37fe1b6036e84bd28f61fb613050871b |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:28:37Z |
| publishDate | 2016-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-37fe1b6036e84bd28f61fb613050871b2022-12-22T03:10:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062016-12-01291293894910.1094/MPMI-08-16-0172-RGABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000S. L. McCraw0D. H. Park1R. Jones2M. A. Bentley3A. Rico4R. G. Ratcliffe5N. J. Kruger6A. Collmer7G. M. Preston8Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.;Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea;Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.;Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.;Departamento de Didáctica de la 9 Matemática y de las Ciencias Experimentales, Faculty of Education and Sport, University of the Basque Country UPV/EHU, Juan Ibañez de Sto. Domingo 1, 01006 Vitoria-Gasteiz, Spain; andDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.;Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.;School of Integrative Plant Science, Section of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, U.S.A.Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, U.K.;The nonprotein amino acid γ-aminobutyric acid (GABA) is the most abundant amino acid in the tomato (Solanum lycopersicum) leaf apoplast and is synthesized by Arabidopsis thaliana in response to infection by the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (hereafter called DC3000). High levels of exogenous GABA have previously been shown to repress the expression of the type III secretion system (T3SS) in DC3000, resulting in reduced elicitation of the hypersensitive response (HR) in the nonhost plant tobacco (Nicotiana tabacum). This study demonstrates that the GABA permease GabP provides the primary mechanism for GABA uptake by DC3000 and that the gabP deletion mutant ΔgabP is insensitive to GABA-mediated repression of T3SS expression. ΔgabP displayed an enhanced ability to elicit the HR in young tobacco leaves and in tobacco plants engineered to produce increased levels of GABA, which supports the hypothesis that GABA uptake via GabP acts to regulate T3SS expression in planta. The observation that P. syringae can be rendered insensitive to GABA through loss of gabP but that gabP is retained by this bacterium suggests that GabP is important for DC3000 in a natural setting, either for nutrition or as a mechanism for regulating gene expression.[Graphic: see text] Copyright © 2016 The Author(s). This is an open access article distributed under the CC BY Attribution 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-16-0172-R |
| spellingShingle | S. L. McCraw D. H. Park R. Jones M. A. Bentley A. Rico R. G. Ratcliffe N. J. Kruger A. Collmer G. M. Preston GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000 Molecular Plant-Microbe Interactions |
| title | GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000 |
| title_full | GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000 |
| title_fullStr | GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000 |
| title_full_unstemmed | GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000 |
| title_short | GABA (γ-Aminobutyric Acid) Uptake Via the GABA Permease GabP Represses Virulence Gene Expression in Pseudomonas syringae pv. tomato DC3000 |
| title_sort | gaba γ aminobutyric acid uptake via the gaba permease gabp represses virulence gene expression in pseudomonas syringae pv tomato dc3000 |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-16-0172-R |
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