Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from <i>Coprinopsis cinerea</i>

Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from <i>Coprinopsis cinerea</i>

A endo-1,4-β-mannanase (CcMan5C) gene was cloned from <i>Coprinopsis cinerea</i> and heterologously expressed in <i>Pichia pastoris</i>, and the recombinant enzyme was purified by Ni-affinity chromatography and identified by matrix-assisted laser desorption/ionization time-of...

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Bibliographic Details
Main Authors: Songling Yan, Baiyun Duan, Cuicui Liu, Guiyou Liu, Liqin Kang, Lei Sun, Lin Yi, Zhenqing Zhang, Zhonghua Liu, Sheng Yuan
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Journal of Fungi
Subjects:
β-mannanase
alkalic thermophilic β-mannanase
resistance to organic solvents
resistance to detergents
storage stability
Online Access:https://www.mdpi.com/2309-608X/9/3/378

Internet

https://www.mdpi.com/2309-608X/9/3/378

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