Comprehensive computational analysis of epigenetic descriptors affecting CRISPR-Cas9 off-target activity

Comprehensive computational analysis of epigenetic descriptors affecting CRISPR-Cas9 off-target activity

Abstract Background A common issue in CRISPR-Cas9 genome editing is off-target activity, which prevents the widespread use of CRISPR-Cas9 in medical applications. Among other factors, primary chromatin structure and epigenetics may influence off-target activity. Methods In this work, we utilize cris...

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Bibliographic Details
Main Authors: Jeffrey K. Mak, Florian Störtz, Peter Minary
Format: Article
Language:English
Published: BMC 2022-12-01
Series:BMC Genomics
Subjects:
CRISPR-Cas9
Off-target activity
Nucleosome
crisprSQL
Gene editing
Machine Learning
Online Access:https://doi.org/10.1186/s12864-022-09012-7

Internet

https://doi.org/10.1186/s12864-022-09012-7

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