Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers

The study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53 watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty SSR markers were shown to be highly polymorphic and were stat...

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Main Author: Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi
Format: Article
Language:English
Published: Indian Society of Plant Breeders 2022-03-01
Series:Electronic Journal of Plant Breeding
Subjects:
Online Access:https://www.ejplantbreeding.org/index.php/EJPB/article/view/4146
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author Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi
author_facet Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi
author_sort Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi
collection DOAJ
description The study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53 watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty SSR markers were shown to be highly polymorphic and were statistically analyzed using Power Marker and NTSYSPc softwares. A total of 33 alleles were generated by polymorphic SSR markers, on an average of 2.6 alleles per loci. Heterozygosity among accessions for individual loci varied from zero to 0.17 (BVWS02306 marker).The gene diversity and PIC values varied between 0.08 to 0.72 and 0.46 to 0.90, respectively and the primer which had the highest gene diversity and PIC values was BVWS02422. The UPGMA-based dendrogram classified all the 53 accessions into two major clusters at 48 per cent similarity. All 41 cultivated watermelon accessions were grouped in cluster I and the remaining 12 accessions from wild types were grouped in cluster II. Thus, this study highlights the importance of molecular markers in the identification of wild and cultivated lines and their exploitation in future breeding programs.
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spelling doaj.art-38d4ef441b064700893c38cb93e2f1212023-04-04T14:51:16ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2022-03-0113116817410.37992/2022.1301.024Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markersKoushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi0Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India *E-Mail: harshahit2001@yahoo.co.inThe study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53 watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty SSR markers were shown to be highly polymorphic and were statistically analyzed using Power Marker and NTSYSPc softwares. A total of 33 alleles were generated by polymorphic SSR markers, on an average of 2.6 alleles per loci. Heterozygosity among accessions for individual loci varied from zero to 0.17 (BVWS02306 marker).The gene diversity and PIC values varied between 0.08 to 0.72 and 0.46 to 0.90, respectively and the primer which had the highest gene diversity and PIC values was BVWS02422. The UPGMA-based dendrogram classified all the 53 accessions into two major clusters at 48 per cent similarity. All 41 cultivated watermelon accessions were grouped in cluster I and the remaining 12 accessions from wild types were grouped in cluster II. Thus, this study highlights the importance of molecular markers in the identification of wild and cultivated lines and their exploitation in future breeding programs.https://www.ejplantbreeding.org/index.php/EJPB/article/view/4146watermelongenetic diversitymicrosatellitejaccard similarity coefficient
spellingShingle Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi
Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
Electronic Journal of Plant Breeding
watermelon
genetic diversity
microsatellite
jaccard similarity coefficient
title Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
title_full Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
title_fullStr Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
title_full_unstemmed Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
title_short Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
title_sort genetic diversity analysis among different horticultural groups of indigenous and exotic citrullus landraces using microsatellite markers
topic watermelon
genetic diversity
microsatellite
jaccard similarity coefficient
url https://www.ejplantbreeding.org/index.php/EJPB/article/view/4146
work_keys_str_mv AT koushiksahaharshawardhanchoudharyjayantajamatiaandadmunshi geneticdiversityanalysisamongdifferenthorticulturalgroupsofindigenousandexoticcitrulluslandracesusingmicrosatellitemarkers