Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers
The study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53 watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty SSR markers were shown to be highly polymorphic and were stat...
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Format: | Article |
Language: | English |
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Indian Society of Plant Breeders
2022-03-01
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Series: | Electronic Journal of Plant Breeding |
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Online Access: | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4146 |
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author | Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi |
author_facet | Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi |
author_sort | Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi |
collection | DOAJ |
description | The study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53
watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty
SSR markers were shown to be highly polymorphic and were statistically analyzed using Power Marker and NTSYSPc
softwares. A total of 33 alleles were generated by polymorphic SSR markers, on an average of 2.6 alleles per loci.
Heterozygosity among accessions for individual loci varied from zero to 0.17 (BVWS02306 marker).The gene diversity
and PIC values varied between 0.08 to 0.72 and 0.46 to 0.90, respectively and the primer which had the highest gene
diversity and PIC values was BVWS02422. The UPGMA-based dendrogram classified all the 53 accessions into
two major clusters at 48 per cent similarity. All 41 cultivated watermelon accessions were grouped in cluster I and
the remaining 12 accessions from wild types were grouped in cluster II. Thus, this study highlights the importance of
molecular markers in the identification of wild and cultivated lines and their exploitation in future breeding programs. |
first_indexed | 2024-04-09T19:34:14Z |
format | Article |
id | doaj.art-38d4ef441b064700893c38cb93e2f121 |
institution | Directory Open Access Journal |
issn | 0975-928X |
language | English |
last_indexed | 2024-04-09T19:34:14Z |
publishDate | 2022-03-01 |
publisher | Indian Society of Plant Breeders |
record_format | Article |
series | Electronic Journal of Plant Breeding |
spelling | doaj.art-38d4ef441b064700893c38cb93e2f1212023-04-04T14:51:16ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2022-03-0113116817410.37992/2022.1301.024Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markersKoushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi0Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India *E-Mail: harshahit2001@yahoo.co.inThe study utilised a total of twenty simple sequence repeat (SSR) markers to investigate the genetic diversity of 53 watermelon accessions from different groups of Citrullus i.e., citroides, lanatus, and colocythis. Twelve of the twenty SSR markers were shown to be highly polymorphic and were statistically analyzed using Power Marker and NTSYSPc softwares. A total of 33 alleles were generated by polymorphic SSR markers, on an average of 2.6 alleles per loci. Heterozygosity among accessions for individual loci varied from zero to 0.17 (BVWS02306 marker).The gene diversity and PIC values varied between 0.08 to 0.72 and 0.46 to 0.90, respectively and the primer which had the highest gene diversity and PIC values was BVWS02422. The UPGMA-based dendrogram classified all the 53 accessions into two major clusters at 48 per cent similarity. All 41 cultivated watermelon accessions were grouped in cluster I and the remaining 12 accessions from wild types were grouped in cluster II. Thus, this study highlights the importance of molecular markers in the identification of wild and cultivated lines and their exploitation in future breeding programs.https://www.ejplantbreeding.org/index.php/EJPB/article/view/4146watermelongenetic diversitymicrosatellitejaccard similarity coefficient |
spellingShingle | Koushik Saha, Harshawardhan Choudhary*, Jayanta Jamatia and A.D Munshi Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers Electronic Journal of Plant Breeding watermelon genetic diversity microsatellite jaccard similarity coefficient |
title | Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers |
title_full | Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers |
title_fullStr | Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers |
title_full_unstemmed | Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers |
title_short | Genetic diversity analysis among different horticultural groups of indigenous and exotic Citrullus landraces using microsatellite markers |
title_sort | genetic diversity analysis among different horticultural groups of indigenous and exotic citrullus landraces using microsatellite markers |
topic | watermelon genetic diversity microsatellite jaccard similarity coefficient |
url | https://www.ejplantbreeding.org/index.php/EJPB/article/view/4146 |
work_keys_str_mv | AT koushiksahaharshawardhanchoudharyjayantajamatiaandadmunshi geneticdiversityanalysisamongdifferenthorticulturalgroupsofindigenousandexoticcitrulluslandracesusingmicrosatellitemarkers |