An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a

An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a

CRISPR/Cas12a technology is used for nucleic acid detection due to its specific recognition function and non-specific single-stranded DNA cleavage activity. Here, we developed a fluorescence visualisation detection method based on PCR and CRISPR/Cas12a approaches. The method was used to detect the n...

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Bibliographic Details
Main Authors: Yuling Wang, Cheng Peng, Lin Ding, Zhixun Su, Xiaoyun Chen, Xiaofu Wang, Meihao Sun, Junfeng Xu
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Foods
Subjects:
T-nos
nucleic acid detection
CRISPR/Cas12a
fluorescence visualisation
PCR
Online Access:https://www.mdpi.com/2304-8158/12/3/615

Internet

https://www.mdpi.com/2304-8158/12/3/615

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