A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents
A liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grai...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2014-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679014000147/type/journal_article |
| _version_ | 1827994624886571008 |
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| author | Kirstine Lykke Nielsen Mette Skou Hedemann Helle Nygaard Lærke Henry Jørgensen Knud Erik Bach Knudsen |
| author_facet | Kirstine Lykke Nielsen Mette Skou Hedemann Helle Nygaard Lærke Henry Jørgensen Knud Erik Bach Knudsen |
| author_sort | Kirstine Lykke Nielsen |
| collection | DOAJ |
| description | A liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grains has received little attention. Six female catheterised pigs were given two white wheat breads with wheat AX or oat BG, two rye breads with ground rye (GR) or intact rye kernels (RK), and a control white wheat bread (WF) on separate occasions in a randomised cross-over design. The amount of available carbohydrate was similar for the five breads but varied in the content of protein. Plasma was collected continuously for 4 h after feeding. Glucose levels in the portal vein were reduced postprandially in response to the AX, GR and RK breads that had high contents of AX compared with WF bread (P < 0·03). AX and RK breads further tended to decrease plasma levels of some lysophosphatidylcholine species (P ≤ 0·10). The abundance of amino acids in plasma correlated with the protein contents in the breads and leucine uptake significantly affected insulin secretion in the mesenteric artery. In conclusion, the present study revealed that concentrated AX in wheat bread had similar positive effects as whole-grain rye bread on glucose and lipid metabolism. |
| first_indexed | 2024-04-10T04:43:49Z |
| format | Article |
| id | doaj.art-38dfc4d745e141c5829bb4a35c1f5f76 |
| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-04-10T04:43:49Z |
| publishDate | 2014-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-38dfc4d745e141c5829bb4a35c1f5f762023-03-09T12:38:43ZengCambridge University PressJournal of Nutritional Science2048-67902014-01-01310.1017/jns.2014.14A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contentsKirstine Lykke Nielsen0Mette Skou Hedemann1Helle Nygaard Lærke2Henry Jørgensen3Knud Erik Bach Knudsen4Department of Forensic Medicine, Forensic Chemistry, Aarhus University, Brendstrupgårdsvej 100, DK-8200 Aarhus N, DenmarkDepartment of Animal Science, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkDepartment of Animal Science, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkDepartment of Animal Science, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkDepartment of Animal Science, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkA liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grains has received little attention. Six female catheterised pigs were given two white wheat breads with wheat AX or oat BG, two rye breads with ground rye (GR) or intact rye kernels (RK), and a control white wheat bread (WF) on separate occasions in a randomised cross-over design. The amount of available carbohydrate was similar for the five breads but varied in the content of protein. Plasma was collected continuously for 4 h after feeding. Glucose levels in the portal vein were reduced postprandially in response to the AX, GR and RK breads that had high contents of AX compared with WF bread (P < 0·03). AX and RK breads further tended to decrease plasma levels of some lysophosphatidylcholine species (P ≤ 0·10). The abundance of amino acids in plasma correlated with the protein contents in the breads and leucine uptake significantly affected insulin secretion in the mesenteric artery. In conclusion, the present study revealed that concentrated AX in wheat bread had similar positive effects as whole-grain rye bread on glucose and lipid metabolism.https://www.cambridge.org/core/product/identifier/S2048679014000147/type/journal_articleArabinoxylanβ-GlucanCatheterised pigsRyeMetabolomics |
| spellingShingle | Kirstine Lykke Nielsen Mette Skou Hedemann Helle Nygaard Lærke Henry Jørgensen Knud Erik Bach Knudsen A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents Journal of Nutritional Science Arabinoxylan β-Glucan Catheterised pigs Rye Metabolomics |
| title | A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents |
| title_full | A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents |
| title_fullStr | A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents |
| title_full_unstemmed | A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents |
| title_short | A metabolomics approach used to profile plasma from portal–arterial pigs revealed differences between breads incurred by dietary fibre and protein contents |
| title_sort | metabolomics approach used to profile plasma from portal arterial pigs revealed differences between breads incurred by dietary fibre and protein contents |
| topic | Arabinoxylan β-Glucan Catheterised pigs Rye Metabolomics |
| url | https://www.cambridge.org/core/product/identifier/S2048679014000147/type/journal_article |
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