Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential
Mycobacterium species exhibit high bioremediation potential for the degradation of polycyclic aromatic hydrocarbons (PAHs) that are significant environmental pollutants. In this study, three Gram-positive, rapidly growing strains (YC-RL4T, MB418T, and HX176T) were isolated from petroleum-contaminate...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1225746/full |
| _version_ | 1797689687494623232 |
|---|---|
| author | Yang Deng Yang Deng Tong Mou Tong Mou Junhuan Wang Jing Su Yanchun Yan Yu-Qin Zhang Yu-Qin Zhang |
| author_facet | Yang Deng Yang Deng Tong Mou Tong Mou Junhuan Wang Jing Su Yanchun Yan Yu-Qin Zhang Yu-Qin Zhang |
| author_sort | Yang Deng |
| collection | DOAJ |
| description | Mycobacterium species exhibit high bioremediation potential for the degradation of polycyclic aromatic hydrocarbons (PAHs) that are significant environmental pollutants. In this study, three Gram-positive, rapidly growing strains (YC-RL4T, MB418T, and HX176T) were isolated from petroleum-contaminated soils and were classified as Mycobacterium within the family Mycobacteriaceae. Genomic average nucleotide identity (ANI; < 95%) and digital DNA–DNA hybridization (dDDH; < 70%) values relative to other Mycobacterium spp. indicated that the strains represented novel species. The morphological, physiological, and chemotaxonomic characteristics of the isolates also supported their affiliation with Mycobacterium and their delineation as novel species. The strains were identified as Mycobacterium adipatum sp. nov. (type strain YC-RL4T = CPCC 205684T = CGMCC 1.62027T), Mycobacterium deserti sp. nov. (type strain MB418T = CPCC 205710T = KCTC 49782T), and Mycobacterium hippophais sp. nov. (type strain HX176T = CPCC 205372T = KCTC 49413T). Genes encoding enzymes involved in PAH degradation and metal resistance were present in the genomes of all three strains. Specifically, genes encoding alpha subunits of aromatic ring-hydroxylating dioxygenases were encoded by the genomes. The genes were also identified as core genes in a pangenomic analysis of the three strains along with 70 phylogenetically related mycobacterial strains that were previously classified as Mycolicibacterium. Notably, strain YC-RL4T could not only utilize phthalates as their sole carbon source for growth, but also convert di-(2-ethylhexyl) phthalate into phthalic acid. These results indicated that strains YC-RL4T, MB418T, and HX176T were important resources with significant bioremediation potential in soils contaminated by PAHs and heavy metals. |
| first_indexed | 2024-03-12T01:49:07Z |
| format | Article |
| id | doaj.art-38e49a2175804a91b646ed16c0e2fa13 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T01:49:07Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-38e49a2175804a91b646ed16c0e2fa132023-09-08T13:47:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12257461225746Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potentialYang Deng0Yang Deng1Tong Mou2Tong Mou3Junhuan Wang4Jing Su5Yanchun Yan6Yu-Qin Zhang7Yu-Qin Zhang8Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaState Key Laboratory of Dao-di Herbs, Beijing, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaState Key Laboratory of Dao-di Herbs, Beijing, ChinaGraduate School of Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaGraduate School of Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaState Key Laboratory of Dao-di Herbs, Beijing, ChinaMycobacterium species exhibit high bioremediation potential for the degradation of polycyclic aromatic hydrocarbons (PAHs) that are significant environmental pollutants. In this study, three Gram-positive, rapidly growing strains (YC-RL4T, MB418T, and HX176T) were isolated from petroleum-contaminated soils and were classified as Mycobacterium within the family Mycobacteriaceae. Genomic average nucleotide identity (ANI; < 95%) and digital DNA–DNA hybridization (dDDH; < 70%) values relative to other Mycobacterium spp. indicated that the strains represented novel species. The morphological, physiological, and chemotaxonomic characteristics of the isolates also supported their affiliation with Mycobacterium and their delineation as novel species. The strains were identified as Mycobacterium adipatum sp. nov. (type strain YC-RL4T = CPCC 205684T = CGMCC 1.62027T), Mycobacterium deserti sp. nov. (type strain MB418T = CPCC 205710T = KCTC 49782T), and Mycobacterium hippophais sp. nov. (type strain HX176T = CPCC 205372T = KCTC 49413T). Genes encoding enzymes involved in PAH degradation and metal resistance were present in the genomes of all three strains. Specifically, genes encoding alpha subunits of aromatic ring-hydroxylating dioxygenases were encoded by the genomes. The genes were also identified as core genes in a pangenomic analysis of the three strains along with 70 phylogenetically related mycobacterial strains that were previously classified as Mycolicibacterium. Notably, strain YC-RL4T could not only utilize phthalates as their sole carbon source for growth, but also convert di-(2-ethylhexyl) phthalate into phthalic acid. These results indicated that strains YC-RL4T, MB418T, and HX176T were important resources with significant bioremediation potential in soils contaminated by PAHs and heavy metals.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1225746/fullMycobacterium adipatumMycobacterium desertiMycobacterium hippophaisgenomebioremediation potential |
| spellingShingle | Yang Deng Yang Deng Tong Mou Tong Mou Junhuan Wang Jing Su Yanchun Yan Yu-Qin Zhang Yu-Qin Zhang Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential Frontiers in Microbiology Mycobacterium adipatum Mycobacterium deserti Mycobacterium hippophais genome bioremediation potential |
| title | Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential |
| title_full | Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential |
| title_fullStr | Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential |
| title_full_unstemmed | Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential |
| title_short | Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential |
| title_sort | characterization of three rapidly growing novel mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential |
| topic | Mycobacterium adipatum Mycobacterium deserti Mycobacterium hippophais genome bioremediation potential |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1225746/full |
| work_keys_str_mv | AT yangdeng characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT yangdeng characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT tongmou characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT tongmou characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT junhuanwang characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT jingsu characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT yanchunyan characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT yuqinzhang characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential AT yuqinzhang characterizationofthreerapidlygrowingnovelmycobacteriumspecieswithsignificantpolycyclicaromatichydrocarbonbioremediationpotential |