RNase If -treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops

Abstract Background RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such as resistance against insects, fungi, viruses, and metabolism manipula...

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Bibliographic Details
Main Authors: Po-Hao Wang, Greg Schulenberg, Shannon Whitlock, Andrew Worden, Ning Zhou, Stephen Novak, Wei Chen
Format: Article
Language:English
Published: BMC 2018-01-01
Series:BMC Biotechnology
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12896-018-0413-6