Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries of heterogeneous quality. Here, we present a rapid...

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Bibliographic Details
Main Authors: Martin Wegner, Valentina Diehl, Verena Bittl, Rahel de Bruyn, Svenja Wiechmann, Yves Matthess, Marie Hebel, Michael GB Hayes, Simone Schaubeck, Christopher Benner, Sven Heinz, Anja Bremm, Ivan Dikic, Andreas Ernst, Manuel Kaulich
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-03-01
Series:eLife
Subjects:
CRISPR/Cas
gRNA library
3Cs technology
genome-wide
DUBs
Doxorubicin
Online Access:https://elifesciences.org/articles/42549

Internet

https://elifesciences.org/articles/42549

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