The Biotechnological Applications of Recombinant Single-Domain Antibodies are Optimized by the C-Terminal Fusion to the EPEA Sequence (C Tag)

The Biotechnological Applications of Recombinant Single-Domain Antibodies are Optimized by the C-Terminal Fusion to the EPEA Sequence (C Tag)

We designed a vector for the bacterial expression of recombinant antibodies fused to a double tag composed of 6xHis and the EPEA amino acid sequence. EPEA sequence (C tag) is tightly bound by a commercial antibody when expressed at the C-term end of a polypeptide. The antigen is released in the pres...

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Bibliographic Details
Main Authors: Selma Djender, Anne Beugnet, Aurelie Schneider, Ario de Marco
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:Antibodies
Subjects:
antibody screening
cytoplasmic antibody expression
immunoprecipitation
nanobody
VHH
tandem affinity purification
Online Access:http://www.mdpi.com/2073-4468/3/2/182

Internet

http://www.mdpi.com/2073-4468/3/2/182

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