Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration
Abstract Background It is known that the medicinal herb Alpinia oxyphylla Miq. is widely used as a remedy for diarrhea as well as the symptoms accompanying hypertension and cerebrovascular disorders. Moreover, it has also been reported that Alpinia oxyphylla Miq. has beneficial effects on anti-senes...
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| Format: | Article |
| Language: | English |
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BMC
2017-03-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1695-2 |
| _version_ | 1818280509603577856 |
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| author | Yung-Ming Chang Hen-Hong Chang Chin-Chuan Tsai Hung-Jen Lin Tsung-Jung Ho Chi-Xin Ye Ping-Ling Chiu Yueh-Sheng Chen Ray-Jade Chen Chih-Yang Huang Chien-Chung Lin |
| author_facet | Yung-Ming Chang Hen-Hong Chang Chin-Chuan Tsai Hung-Jen Lin Tsung-Jung Ho Chi-Xin Ye Ping-Ling Chiu Yueh-Sheng Chen Ray-Jade Chen Chih-Yang Huang Chien-Chung Lin |
| author_sort | Yung-Ming Chang |
| collection | DOAJ |
| description | Abstract Background It is known that the medicinal herb Alpinia oxyphylla Miq. is widely used as a remedy for diarrhea as well as the symptoms accompanying hypertension and cerebrovascular disorders. Moreover, it has also been reported that Alpinia oxyphylla Miq. has beneficial effects on anti-senescence and neuro-protection. This study focuses on the molecular mechanisms by which the Alpinia oxyphylla Miq. fruits promote neuron regeneration. Methods A piece of silicone rubber was guided across a 15 mm gap in the sciatic nerve of a rat. This nerve gap was then filled with various doses of Alpinia oxyphylla Miq. fruits to assess their regenerative effect on damaged nerves. Further, we investigated the role of Alpinia oxyphylla Miq. fruits in RSC96 Schwann cell proliferation. Results Our current results showed that treatment with the extract of Alpinia oxyphylla Miq. fruits triggers the phosphorylated insulin-like growth factor-1 receptor- phosphatidylinositol 3-kinase/serine-threonine kinase pathway, and up-regulated the proliferating cell nuclear antigen in a dose-dependent manner. Cell cycle analysis on RSC96 Schwann cells showed that, after exposure to Alpinia oxyphylla Miq. fruit extract, the transition from the first gap phase to the synthesis phase occurs in 12–18 h. The expression of the cell cycle regulatory proteins cyclin D1, cyclin E and cyclin A increased in a dose-dependent manner. Transfection with a small interfering RNA blocked the expression of phosphatidylinositol 3-kinase and induced down-regulation both on the mRNA and protein levels, which resulted in a reduction of the expression of the survival factor B-cell lymphoma 2. Conclusion We provide positive results that demonstrate that Alpinia oxyphylla Miq. fruits facilitate the survival and proliferation of RSC96 cells via insulin-like growth factor-1 signaling. |
| first_indexed | 2024-12-12T23:50:22Z |
| format | Article |
| id | doaj.art-395f200fbe094aa492e2d53d17ba725b |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-12T23:50:22Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-395f200fbe094aa492e2d53d17ba725b2022-12-22T00:06:43ZengBMCBMC Complementary and Alternative Medicine1472-68822017-03-011711910.1186/s12906-017-1695-2Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regenerationYung-Ming Chang0Hen-Hong Chang1Chin-Chuan Tsai2Hung-Jen Lin3Tsung-Jung Ho4Chi-Xin Ye5Ping-Ling Chiu6Yueh-Sheng Chen7Ray-Jade Chen8Chih-Yang Huang9Chien-Chung Lin10The School of Chinese Medicine for Post-Baccalaureate, I-Shou UniversityResearch Center for Chinese Medicine & Acupuncture, China Medical UniversityThe School of Chinese Medicine for Post-Baccalaureate, I-Shou UniversityDivision of Chinese Internal Medicine, China Medical University HospitalSchool of Chinese Medicine, China Medical UniversityGraduate Institute of Basic Medical Science, China Medical University1PT Biotechnology Co., Ltd.School of Chinese Medicine, China Medical UniversityDepartment of Surgery, School of Medicine, College of Medicine, Taipei Medical UniversitySchool of Chinese Medicine, China Medical UniversityOrthopaedic Department Armed Forces General HospitalAbstract Background It is known that the medicinal herb Alpinia oxyphylla Miq. is widely used as a remedy for diarrhea as well as the symptoms accompanying hypertension and cerebrovascular disorders. Moreover, it has also been reported that Alpinia oxyphylla Miq. has beneficial effects on anti-senescence and neuro-protection. This study focuses on the molecular mechanisms by which the Alpinia oxyphylla Miq. fruits promote neuron regeneration. Methods A piece of silicone rubber was guided across a 15 mm gap in the sciatic nerve of a rat. This nerve gap was then filled with various doses of Alpinia oxyphylla Miq. fruits to assess their regenerative effect on damaged nerves. Further, we investigated the role of Alpinia oxyphylla Miq. fruits in RSC96 Schwann cell proliferation. Results Our current results showed that treatment with the extract of Alpinia oxyphylla Miq. fruits triggers the phosphorylated insulin-like growth factor-1 receptor- phosphatidylinositol 3-kinase/serine-threonine kinase pathway, and up-regulated the proliferating cell nuclear antigen in a dose-dependent manner. Cell cycle analysis on RSC96 Schwann cells showed that, after exposure to Alpinia oxyphylla Miq. fruit extract, the transition from the first gap phase to the synthesis phase occurs in 12–18 h. The expression of the cell cycle regulatory proteins cyclin D1, cyclin E and cyclin A increased in a dose-dependent manner. Transfection with a small interfering RNA blocked the expression of phosphatidylinositol 3-kinase and induced down-regulation both on the mRNA and protein levels, which resulted in a reduction of the expression of the survival factor B-cell lymphoma 2. Conclusion We provide positive results that demonstrate that Alpinia oxyphylla Miq. fruits facilitate the survival and proliferation of RSC96 cells via insulin-like growth factor-1 signaling.http://link.springer.com/article/10.1186/s12906-017-1695-2Alpinia oxyphylla Miq. fruit extractIGF-1nerve regenerationproliferationRSC96 Schwann cell |
| spellingShingle | Yung-Ming Chang Hen-Hong Chang Chin-Chuan Tsai Hung-Jen Lin Tsung-Jung Ho Chi-Xin Ye Ping-Ling Chiu Yueh-Sheng Chen Ray-Jade Chen Chih-Yang Huang Chien-Chung Lin Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration BMC Complementary and Alternative Medicine Alpinia oxyphylla Miq. fruit extract IGF-1 nerve regeneration proliferation RSC96 Schwann cell |
| title | Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration |
| title_full | Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration |
| title_fullStr | Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration |
| title_full_unstemmed | Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration |
| title_short | Alpinia oxyphylla Miq. fruit extract activates IGFR-PI3K/Akt signaling to induce Schwann cell proliferation and sciatic nerve regeneration |
| title_sort | alpinia oxyphylla miq fruit extract activates igfr pi3k akt signaling to induce schwann cell proliferation and sciatic nerve regeneration |
| topic | Alpinia oxyphylla Miq. fruit extract IGF-1 nerve regeneration proliferation RSC96 Schwann cell |
| url | http://link.springer.com/article/10.1186/s12906-017-1695-2 |
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