The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion
The purinergic 2X7 (P2X7) receptor expressed in satellite glial cells (SGCs) is involved in the inflammatory response, and transient receptor potential vanilloid 1 (TRPV1) participates in the process of neurogenic inflammation, such as that in diabetic neuropathic pain (DNP) and peripheral neuralgia...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2021.663649/full |
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| author | Anhui Wang Xiangchao Shi Ruoyang Yu Bao Qiao Runan Yang Changshui Xu Changshui Xu |
| author_facet | Anhui Wang Xiangchao Shi Ruoyang Yu Bao Qiao Runan Yang Changshui Xu Changshui Xu |
| author_sort | Anhui Wang |
| collection | DOAJ |
| description | The purinergic 2X7 (P2X7) receptor expressed in satellite glial cells (SGCs) is involved in the inflammatory response, and transient receptor potential vanilloid 1 (TRPV1) participates in the process of neurogenic inflammation, such as that in diabetic neuropathic pain (DNP) and peripheral neuralgia. The main purpose of this study was to explore the role of the P2X7 receptor in DNP hypersensitivity mediated by TRPV1 in the rat and its possible mechanism. A rat model of type 2 diabetes mellitus-related neuropathic pain (NPP) named the DNP rat model was established in this study. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of DNP rats were increased after intrathecal injection of the P2X7 receptor antagonist A438079, and the mRNA and protein levels of TRPV1 in the dorsal root ganglion (DRG) were decreased in DNP rats treated with A438079 compared to untreated DNP rats; in addition, A438079 also decreased the phosphorylation of p38 and extracellular signal-regulated kinase 1/2 (ERK1/2) in the DNP group. Based on these results, the P2X7 receptor might be involved in DNP mediated by TRPV1. |
| first_indexed | 2024-12-17T05:11:21Z |
| format | Article |
| id | doaj.art-395fbc78dd9745f09c4804a1467928c8 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-17T05:11:21Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-395fbc78dd9745f09c4804a1467928c82022-12-21T22:02:14ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992021-06-011410.3389/fnmol.2021.663649663649The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root GanglionAnhui Wang0Xiangchao Shi1Ruoyang Yu2Bao Qiao3Runan Yang4Changshui Xu5Changshui Xu6Department of Physiology, Basic Medical College of Nanchang University, Nanchang, ChinaMedical Department, Queen Mary School, Nanchang University, Nanchang, ChinaMedical Department, Queen Mary School, Nanchang University, Nanchang, ChinaMedical Department, Queen Mary School, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College of Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College of Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, ChinaThe purinergic 2X7 (P2X7) receptor expressed in satellite glial cells (SGCs) is involved in the inflammatory response, and transient receptor potential vanilloid 1 (TRPV1) participates in the process of neurogenic inflammation, such as that in diabetic neuropathic pain (DNP) and peripheral neuralgia. The main purpose of this study was to explore the role of the P2X7 receptor in DNP hypersensitivity mediated by TRPV1 in the rat and its possible mechanism. A rat model of type 2 diabetes mellitus-related neuropathic pain (NPP) named the DNP rat model was established in this study. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of DNP rats were increased after intrathecal injection of the P2X7 receptor antagonist A438079, and the mRNA and protein levels of TRPV1 in the dorsal root ganglion (DRG) were decreased in DNP rats treated with A438079 compared to untreated DNP rats; in addition, A438079 also decreased the phosphorylation of p38 and extracellular signal-regulated kinase 1/2 (ERK1/2) in the DNP group. Based on these results, the P2X7 receptor might be involved in DNP mediated by TRPV1.https://www.frontiersin.org/articles/10.3389/fnmol.2021.663649/fulldiabetic neuropathic painP2X7 receptorsTRPV1dorsal root ganglionsatellite glial cellsneurons |
| spellingShingle | Anhui Wang Xiangchao Shi Ruoyang Yu Bao Qiao Runan Yang Changshui Xu Changshui Xu The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion Frontiers in Molecular Neuroscience diabetic neuropathic pain P2X7 receptors TRPV1 dorsal root ganglion satellite glial cells neurons |
| title | The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion |
| title_full | The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion |
| title_fullStr | The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion |
| title_full_unstemmed | The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion |
| title_short | The P2X7 Receptor Is Involved in Diabetic Neuropathic Pain Hypersensitivity Mediated by TRPV1 in the Rat Dorsal Root Ganglion |
| title_sort | p2x7 receptor is involved in diabetic neuropathic pain hypersensitivity mediated by trpv1 in the rat dorsal root ganglion |
| topic | diabetic neuropathic pain P2X7 receptors TRPV1 dorsal root ganglion satellite glial cells neurons |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2021.663649/full |
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