Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review

Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying expression levels of targeted genes during various biological processes in numerous areas of clinical and biological research. Selection of appropriate reference genes for RT-qPCR normaliza...

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Bibliographic Details
Main Authors: Jing Lü, Chunxiao Yang, Youjun Zhang, Huipeng Pan
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-11-01
Series:Frontiers in Physiology
Subjects:
RT-qPCR
reference genes
SYBR green method
experimental factors
analysis tools
Online Access:https://www.frontiersin.org/article/10.3389/fphys.2018.01560/full

Internet

https://www.frontiersin.org/article/10.3389/fphys.2018.01560/full

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