Single-neuron RNA-Seq: technical feasibility and reproducibility

Single-neuron RNA-Seq: technical feasibility and reproducibility

Understanding brain function involves improved knowledge about how the genome specifies such a large diversity of neuronal types. Transcriptome analysis of single neurons has been previously described using gene expression microarrays. Using high-throughput transcriptome sequencing (RNA-Seq), we hav...

Full description

Bibliographic Details
Main Authors: Shenfeng eQiu, Shujun eLuo, Oleg eEvgrafov, Robin eLi, Gary P Schroth, Pat eLevitt, James A Knowles, Kai eWang
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-07-01
Series:Frontiers in Genetics
Subjects:
Electrophysiology
Gene Expression
Transcriptome
cell culture
Neuron
RNA-Seq
Online Access:http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00124/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00124/full

Similar Items