Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis
Alcoholic fatty liver disease (AFLD) is characterized by an abnormal accumulation of lipid droplets (LDs) in the liver. Here, we explore the composition of hepatic LDs in a rat model of AFLD. Five to seven weeks of alcohol consumption led to significant increases in hepatic triglyceride mass, along...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/7/12/230 |
| _version_ | 1827835154693881856 |
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| author | Laura Listenberger Elizabeth Townsend Cassandra Rickertsen Anastasia Hains Elizabeth Brown Emily G. Inwards Angela K. Stoeckman Mitchell P. Matis Rebecca S. Sampathkumar Natalia A. Osna Kusum K. Kharbanda |
| author_facet | Laura Listenberger Elizabeth Townsend Cassandra Rickertsen Anastasia Hains Elizabeth Brown Emily G. Inwards Angela K. Stoeckman Mitchell P. Matis Rebecca S. Sampathkumar Natalia A. Osna Kusum K. Kharbanda |
| author_sort | Laura Listenberger |
| collection | DOAJ |
| description | Alcoholic fatty liver disease (AFLD) is characterized by an abnormal accumulation of lipid droplets (LDs) in the liver. Here, we explore the composition of hepatic LDs in a rat model of AFLD. Five to seven weeks of alcohol consumption led to significant increases in hepatic triglyceride mass, along with increases in LD number and size. Additionally, hepatic LDs from rats with early alcoholic liver injury show a decreased ratio of surface phosphatidylcholine (PC) to phosphatidylethanolamine (PE). This occurred in parallel with an increase in the LD association of perilipin 2, a prominent LD protein. To determine if changes to the LD phospholipid composition contributed to differences in protein association with LDs, we constructed liposomes that modeled the LD PC:PE ratios in AFLD and control rats. Reducing the ratio of PC to PE increased the binding of perilipin 2 to liposomes in an in vitro experiment. Moreover, we decreased the ratio of LD PC:PE in NIH 3T3 and AML12 cells by culturing these cells in choline-deficient media. We again detected increased association of specific LD proteins, including perilipin 2. Taken together, our experiments suggest an important link between LD phospholipids, protein composition, and lipid accumulation. |
| first_indexed | 2024-03-12T06:04:35Z |
| format | Article |
| id | doaj.art-398711a161bc4946983942c65349ea35 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:04:35Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-398711a161bc4946983942c65349ea352023-09-03T03:46:28ZengMDPI AGCells2073-44092018-11-0171223010.3390/cells7120230cells7120230Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and SteatosisLaura Listenberger0Elizabeth Townsend1Cassandra Rickertsen2Anastasia Hains3Elizabeth Brown4Emily G. Inwards5Angela K. Stoeckman6Mitchell P. Matis7Rebecca S. Sampathkumar8Natalia A. Osna9Kusum K. Kharbanda10Departments of Biology and Chemistry, St. Olaf College, Northfield, MN 55057, USADepartments of Biology and Chemistry, St. Olaf College, Northfield, MN 55057, USADepartments of Biology and Chemistry, St. Olaf College, Northfield, MN 55057, USADepartments of Biology and Chemistry, St. Olaf College, Northfield, MN 55057, USADepartments of Biology and Chemistry, St. Olaf College, Northfield, MN 55057, USADepartment of Chemistry, Bethel University, St. Paul, MN 55112, USADepartment of Chemistry, Bethel University, St. Paul, MN 55112, USAResearch Service, VA Nebraska-Western Iowa Health Care System, Omaha, NE and Departments of Internal Medicine and Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68105, USAResearch Service, VA Nebraska-Western Iowa Health Care System, Omaha, NE and Departments of Internal Medicine and Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68105, USAResearch Service, VA Nebraska-Western Iowa Health Care System, Omaha, NE and Departments of Internal Medicine and Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68105, USAResearch Service, VA Nebraska-Western Iowa Health Care System, Omaha, NE and Departments of Internal Medicine and Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68105, USAAlcoholic fatty liver disease (AFLD) is characterized by an abnormal accumulation of lipid droplets (LDs) in the liver. Here, we explore the composition of hepatic LDs in a rat model of AFLD. Five to seven weeks of alcohol consumption led to significant increases in hepatic triglyceride mass, along with increases in LD number and size. Additionally, hepatic LDs from rats with early alcoholic liver injury show a decreased ratio of surface phosphatidylcholine (PC) to phosphatidylethanolamine (PE). This occurred in parallel with an increase in the LD association of perilipin 2, a prominent LD protein. To determine if changes to the LD phospholipid composition contributed to differences in protein association with LDs, we constructed liposomes that modeled the LD PC:PE ratios in AFLD and control rats. Reducing the ratio of PC to PE increased the binding of perilipin 2 to liposomes in an in vitro experiment. Moreover, we decreased the ratio of LD PC:PE in NIH 3T3 and AML12 cells by culturing these cells in choline-deficient media. We again detected increased association of specific LD proteins, including perilipin 2. Taken together, our experiments suggest an important link between LD phospholipids, protein composition, and lipid accumulation.https://www.mdpi.com/2073-4409/7/12/230lipid dropletsperilipinsphosphatidylcholinephosphatidylethanolaminesteatohepatitis |
| spellingShingle | Laura Listenberger Elizabeth Townsend Cassandra Rickertsen Anastasia Hains Elizabeth Brown Emily G. Inwards Angela K. Stoeckman Mitchell P. Matis Rebecca S. Sampathkumar Natalia A. Osna Kusum K. Kharbanda Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis Cells lipid droplets perilipins phosphatidylcholine phosphatidylethanolamine steatohepatitis |
| title | Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis |
| title_full | Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis |
| title_fullStr | Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis |
| title_full_unstemmed | Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis |
| title_short | Decreasing Phosphatidylcholine on the Surface of the Lipid Droplet Correlates with Altered Protein Binding and Steatosis |
| title_sort | decreasing phosphatidylcholine on the surface of the lipid droplet correlates with altered protein binding and steatosis |
| topic | lipid droplets perilipins phosphatidylcholine phosphatidylethanolamine steatohepatitis |
| url | https://www.mdpi.com/2073-4409/7/12/230 |
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