Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR

Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR

Here, a method using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER) for miRNAs quantification was established. The strategy has two steps: (1) ligation of two DNA probes specifically hybridize to target miRNA and (2) qPCR amplifying the ligated probe....

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Bibliographic Details
Main Authors: Xinshu Qin, Xingyu Wang, Ke Xu, Yi Zhang, Hongye Tian, Yinglei Li, Bangran Qi, Xingbin Yang
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: Oligonucleotides: Diagnostics and Biosensors
molecular diagnosis
miRNA quantification
ligation
qPCR
SplintR ligase
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253122003365

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http://www.sciencedirect.com/science/article/pii/S2162253122003365

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