High-Resolution Confocal Fluorescence Imaging of Serine Hydrolase Activity in Cryosections – Application to Glioma Brain Unveils Activity Hotspots Originating from Tumor-Associated Neutrophils

High-Resolution Confocal Fluorescence Imaging of Serine Hydrolase Activity in Cryosections – Application to Glioma Brain Unveils Activity Hotspots Originating from Tumor-Associated Neutrophils

Abstract Background Serine hydrolases (SHs) are a functionally diverse family of enzymes playing pivotal roles in health and disease and have emerged as important therapeutic targets in many clinical conditions. Activity-based protein profiling (ABPP) using fluorophosphonate (FP) probes has been a p...

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Bibliographic Details
Main Authors: Niina Aaltonen, Prosanta K. Singha, Hermina Jakupović, Thomas Wirth, Haritha Samaranayake, Sanna Pasonen-Seppänen, Kirsi Rilla, Markku Varjosalo, Laura E. Edgington-Mitchell, Paulina Kasperkiewicz, Marcin Drag, Sara Kälvälä, Eemeli Moisio, Juha R. Savinainen, Jarmo T. Laitinen
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Biological Procedures Online
Subjects:
Activity-based protein profiling (ABPP)
Brain cryosection
Glioblastoma multiforme (GBM)
Immunohistochemistry
Serine hydrolase activity
Neutrophil serine protease (NSP)
Online Access:http://link.springer.com/article/10.1186/s12575-020-00118-4

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http://link.springer.com/article/10.1186/s12575-020-00118-4

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