Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp.
This is the first study to examine the effects of in vitro digestion on biological activities of <i>Sargassum</i> spp., a broadly known brown seaweed for therapeutic potential. Three fractions (F1–F3) were obtained from hexane extract by column chromatography. Under in vitro simulated di...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2307 |
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| author | Sovannary Un Nguyen Van Quan La Hoang Anh Vu Quang Lam Akiyoshi Takami Tran Dang Khanh Tran Dang Xuan |
| author_facet | Sovannary Un Nguyen Van Quan La Hoang Anh Vu Quang Lam Akiyoshi Takami Tran Dang Khanh Tran Dang Xuan |
| author_sort | Sovannary Un |
| collection | DOAJ |
| description | This is the first study to examine the effects of in vitro digestion on biological activities of <i>Sargassum</i> spp., a broadly known brown seaweed for therapeutic potential. Three fractions (F1–F3) were obtained from hexane extract by column chromatography. Under in vitro simulated digestion, the anti-α-amylase capacity of F1 in oral and intestinal phases increases, while it significantly decreases in the gastric phase. The α-amylase inhibition of F2 promotes throughout all digestive stages while the activity of F3 significantly reduces. The cytotoxic activity of F1 against U266 cell-line accelerates over the oral, gastric, and intestinal stages. The fractions F2 and F3 exhibited the declined cytotoxic potentialities in oral and gastric phases, but they were strengthened under intestinal condition. Palmitic acid and fucosterol may play an active role in antidiabetic and cytotoxic activity against multiple myeloma U266 cell line of <i>Sargassum</i> spp. However, the involvement of other phytochemicals in the seaweed should be further investigated. |
| first_indexed | 2024-03-09T11:35:44Z |
| format | Article |
| id | doaj.art-39d5d73d3782498bb257f63321b6824d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:35:44Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-39d5d73d3782498bb257f63321b6824d2023-11-30T23:42:27ZengMDPI AGMolecules1420-30492022-04-01277230710.3390/molecules27072307Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp.Sovannary Un0Nguyen Van Quan1La Hoang Anh2Vu Quang Lam3Akiyoshi Takami4Tran Dang Khanh5Tran Dang Xuan6Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanTransdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanTransdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanDivision of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, JapanDivision of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, JapanAgricultural Genetics Institute, Pham Van Dong Street, Hanoi 122000, VietnamTransdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, JapanThis is the first study to examine the effects of in vitro digestion on biological activities of <i>Sargassum</i> spp., a broadly known brown seaweed for therapeutic potential. Three fractions (F1–F3) were obtained from hexane extract by column chromatography. Under in vitro simulated digestion, the anti-α-amylase capacity of F1 in oral and intestinal phases increases, while it significantly decreases in the gastric phase. The α-amylase inhibition of F2 promotes throughout all digestive stages while the activity of F3 significantly reduces. The cytotoxic activity of F1 against U266 cell-line accelerates over the oral, gastric, and intestinal stages. The fractions F2 and F3 exhibited the declined cytotoxic potentialities in oral and gastric phases, but they were strengthened under intestinal condition. Palmitic acid and fucosterol may play an active role in antidiabetic and cytotoxic activity against multiple myeloma U266 cell line of <i>Sargassum</i> spp. However, the involvement of other phytochemicals in the seaweed should be further investigated.https://www.mdpi.com/1420-3049/27/7/2307<i>Sargassum</i> spp.α-amylase inhibitoranti-diabeticcytotoxicityU266 cell linemultiple myeloma |
| spellingShingle | Sovannary Un Nguyen Van Quan La Hoang Anh Vu Quang Lam Akiyoshi Takami Tran Dang Khanh Tran Dang Xuan Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp. Molecules <i>Sargassum</i> spp. α-amylase inhibitor anti-diabetic cytotoxicity U266 cell line multiple myeloma |
| title | Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp. |
| title_full | Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp. |
| title_fullStr | Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp. |
| title_full_unstemmed | Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp. |
| title_short | Effects of In Vitro Digestion on Anti-α-Amylase and Cytotoxic Potentials of <i>Sargassum</i> spp. |
| title_sort | effects of in vitro digestion on anti α amylase and cytotoxic potentials of i sargassum i spp |
| topic | <i>Sargassum</i> spp. α-amylase inhibitor anti-diabetic cytotoxicity U266 cell line multiple myeloma |
| url | https://www.mdpi.com/1420-3049/27/7/2307 |
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