Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations

Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations

Summary: microRNAs (miRNAs) have unique gene regulatory effects in different neuronal subpopulations. Here, we describe a protocol to identify neuronal subtype-specific effects of a miRNA in murine motor neuron subpopulations. We detail the preparation of primary mouse spinal tissue for single cell...

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Bibliographic Details
Main Authors: Neal D. Amin, Gokhan Senturk, Marito Hayashi, Shawn P. Driscoll, Samuel L. Pfaff
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Developmental biology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000107

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http://www.sciencedirect.com/science/article/pii/S2666166722000107

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