Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.
Data suggest that clinical applications of human induced pluripotent stem cells (hiPSCs) will be realized. Nonetheless, clinical applications will require hiPSCs that are free of exogenous DNA and that can be manufactured through Good Manufacturing Practice (GMP). Optimally, derivation of hiPSCs sho...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3981795?pdf=render |
| _version_ | 1818022022841630720 |
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| author | Jens Durruthy-Durruthy Sharon F Briggs Jason Awe Cyril Y Ramathal Saravanan Karumbayaram Patrick C Lee Julia D Heidmann Amander Clark Ioannis Karakikes Kyle M Loh Joseph C Wu Andrew R Hoffman James Byrne Renee A Reijo Pera Vittorio Sebastiano |
| author_facet | Jens Durruthy-Durruthy Sharon F Briggs Jason Awe Cyril Y Ramathal Saravanan Karumbayaram Patrick C Lee Julia D Heidmann Amander Clark Ioannis Karakikes Kyle M Loh Joseph C Wu Andrew R Hoffman James Byrne Renee A Reijo Pera Vittorio Sebastiano |
| author_sort | Jens Durruthy-Durruthy |
| collection | DOAJ |
| description | Data suggest that clinical applications of human induced pluripotent stem cells (hiPSCs) will be realized. Nonetheless, clinical applications will require hiPSCs that are free of exogenous DNA and that can be manufactured through Good Manufacturing Practice (GMP). Optimally, derivation of hiPSCs should be rapid and efficient in order to minimize manipulations, reduce potential for accumulation of mutations and minimize financial costs. Previous studies reported the use of modified synthetic mRNAs to reprogram fibroblasts to a pluripotent state. Here, we provide an optimized, fully chemically defined and feeder-free protocol for the derivation of hiPSCs using synthetic mRNAs. The protocol results in derivation of fully reprogrammed hiPSC lines from adult dermal fibroblasts in less than two weeks. The hiPSC lines were successfully tested for their identity, purity, stability and safety at a GMP facility and cryopreserved. To our knowledge, as a proof of principle, these are the first integration-free iPSCs lines that were reproducibly generated through synthetic mRNA reprogramming that could be putatively used for clinical purposes. |
| first_indexed | 2024-04-14T08:25:54Z |
| format | Article |
| id | doaj.art-39f295ca86764194bc0cca198df380ff |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:25:54Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-39f295ca86764194bc0cca198df380ff2022-12-22T02:04:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9423110.1371/journal.pone.0094231Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions.Jens Durruthy-DurruthySharon F BriggsJason AweCyril Y RamathalSaravanan KarumbayaramPatrick C LeeJulia D HeidmannAmander ClarkIoannis KarakikesKyle M LohJoseph C WuAndrew R HoffmanJames ByrneRenee A Reijo PeraVittorio SebastianoData suggest that clinical applications of human induced pluripotent stem cells (hiPSCs) will be realized. Nonetheless, clinical applications will require hiPSCs that are free of exogenous DNA and that can be manufactured through Good Manufacturing Practice (GMP). Optimally, derivation of hiPSCs should be rapid and efficient in order to minimize manipulations, reduce potential for accumulation of mutations and minimize financial costs. Previous studies reported the use of modified synthetic mRNAs to reprogram fibroblasts to a pluripotent state. Here, we provide an optimized, fully chemically defined and feeder-free protocol for the derivation of hiPSCs using synthetic mRNAs. The protocol results in derivation of fully reprogrammed hiPSC lines from adult dermal fibroblasts in less than two weeks. The hiPSC lines were successfully tested for their identity, purity, stability and safety at a GMP facility and cryopreserved. To our knowledge, as a proof of principle, these are the first integration-free iPSCs lines that were reproducibly generated through synthetic mRNA reprogramming that could be putatively used for clinical purposes.http://europepmc.org/articles/PMC3981795?pdf=render |
| spellingShingle | Jens Durruthy-Durruthy Sharon F Briggs Jason Awe Cyril Y Ramathal Saravanan Karumbayaram Patrick C Lee Julia D Heidmann Amander Clark Ioannis Karakikes Kyle M Loh Joseph C Wu Andrew R Hoffman James Byrne Renee A Reijo Pera Vittorio Sebastiano Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. PLoS ONE |
| title | Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. |
| title_full | Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. |
| title_fullStr | Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. |
| title_full_unstemmed | Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. |
| title_short | Rapid and efficient conversion of integration-free human induced pluripotent stem cells to GMP-grade culture conditions. |
| title_sort | rapid and efficient conversion of integration free human induced pluripotent stem cells to gmp grade culture conditions |
| url | http://europepmc.org/articles/PMC3981795?pdf=render |
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