PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency

PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency

VP64 is the smallest transactivation domain that can be packaged together with the sgRNA into a single adeno-associated virus (AAV) vector. However, VP64-based CRISPRa often exerts modest activation to the target gene when only one sgRNA is used. Herein, we used PAM-flexible dual base editor-mediate...

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Bibliographic Details
Main Authors: Cia-Hin Lau, Siping Huang, Raymond H.W. Lam, Chung Tin
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
adeno-associated virus
base editing
dual base editor
nCas9-NG
positive feedback
SpRY
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050122000705

Internet

http://www.sciencedirect.com/science/article/pii/S2329050122000705

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