A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method for <i>Flammulina filiformis</i>

A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method for <i>Flammulina filiformis</i>

CRISPR/Cas9 systems were established in some edible fungi based on in vivo expressed Cas9 and guide RNA. Compared with those systems, the in vitro assembled Cas9 and sgRNA ribonucleoprotein complexes (RNPs) have more advantages, but only a few examples were reported, and the editing efficiency is re...

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Bibliographic Details
Main Authors: Jianyu Liu, Haiyang Cui, Ruijuan Wang, Zhen Xu, Hailong Yu, Chunyan Song, Huan Lu, Qiaozhen Li, Danrun Xing, Qi Tan, Weiming Sun, Gen Zou, Xiaodong Shang
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Journal of Fungi
Subjects:
<i>Flammulina filiformis</i>
CRISPR/Cas9
genomic editing
RNPs
<i>pyrG</i>
Online Access:https://www.mdpi.com/2309-608X/8/10/1000

Internet

https://www.mdpi.com/2309-608X/8/10/1000

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