Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives
The objective was to evaluate the effectiveness of lactic acid bacteria (LAB) inoculants and chemical additives on the fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility of total mixed ration (TMR) silage. Total mixed ration (568 g/kg dry matter (DM)) was...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2206422 |
| _version_ | 1827276559835201536 |
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| author | Siran Wang Guanjun Zhang Jie Zhao Zhihao Dong Junfeng Li Tao Shao |
| author_facet | Siran Wang Guanjun Zhang Jie Zhao Zhihao Dong Junfeng Li Tao Shao |
| author_sort | Siran Wang |
| collection | DOAJ |
| description | The objective was to evaluate the effectiveness of lactic acid bacteria (LAB) inoculants and chemical additives on the fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility of total mixed ration (TMR) silage. Total mixed ration (568 g/kg dry matter (DM)) was ensiled with six experimental treatments: (1) no additives (control); (2) Lactobacillus buchneri (LB; applied at 1 × 106 cfu/g fresh weight (FW)); (3) Lactobacillus casei (LC; applied at 1 × 106 cfu/g FW); (4) calcium propionate (CAP; applied at 0.5% FW); (5) sodium diacetate (SD; applied at 0.5% FW); (6) potassium sorbate (PS; applied at 0.1% FW). All silos (18 L) were opened for fermentation quality, in vitro gas production kinetics and digestibility analysis after 90 days of ensiling, and then subjected to aerobic stability test for 14 days. All the TMR silage was well-preserved with low pH (4.36 ∼ 4.66) and acceptable levels of butyric acid (1.02 ∼ 2.51 g/kg DM) and ammonia nitrogen (86.3 ∼ 107 g/kg total nitrogen). All the groups were steady during 14 days of aerobic exposure, while SD group was more stable with lower (p < 0.05) yeast (4.60 vs. 5.17 ∼ 5.77 log10 cfu/g FW) and mould (3.33 vs. 4.12 ∼ 4.64 log10 cfu/g FW) populations than other treated groups on day 14. Moreover, SD group had the highest (p < 0.05) in vitro digestibility of dry matter (67.8 vs. 56.6 ∼ 63.5%) and neutral detergent fibre (61.7 vs. 50.5 ∼ 57.4%) among all groups. Overall, SD is recommended as additive to improve fermentation quality, in vitro ruminal digestibility, and aerobic stability of TMR silage.HIGHLIGHTS After 14 days of aerobic exposure, the yeast and mould populations in TMR silage can be efficiently inhibited by sodium diacetate treatment. Sodium diacetate treatment evidently enhanced in vitro digestibility of dry matter and neutral detergent fibre compared to other treatments. Sodium diacetate is recommended as additive to improve fermentation quality, in vitro ruminal digestibility, and aerobic stability of TMR silage. |
| first_indexed | 2024-03-08T16:59:45Z |
| format | Article |
| id | doaj.art-3a27999073cb487784ec55dd87ed93a1 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-04-24T07:00:48Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-3a27999073cb487784ec55dd87ed93a12024-04-22T08:52:28ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-0122143044110.1080/1828051X.2023.22064222206422Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additivesSiran Wang0Guanjun Zhang1Jie Zhao2Zhihao Dong3Junfeng Li4Tao Shao5Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural UniversityInstitute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural UniversityInstitute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural UniversityInstitute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural UniversityInstitute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural UniversityInstitute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural UniversityThe objective was to evaluate the effectiveness of lactic acid bacteria (LAB) inoculants and chemical additives on the fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility of total mixed ration (TMR) silage. Total mixed ration (568 g/kg dry matter (DM)) was ensiled with six experimental treatments: (1) no additives (control); (2) Lactobacillus buchneri (LB; applied at 1 × 106 cfu/g fresh weight (FW)); (3) Lactobacillus casei (LC; applied at 1 × 106 cfu/g FW); (4) calcium propionate (CAP; applied at 0.5% FW); (5) sodium diacetate (SD; applied at 0.5% FW); (6) potassium sorbate (PS; applied at 0.1% FW). All silos (18 L) were opened for fermentation quality, in vitro gas production kinetics and digestibility analysis after 90 days of ensiling, and then subjected to aerobic stability test for 14 days. All the TMR silage was well-preserved with low pH (4.36 ∼ 4.66) and acceptable levels of butyric acid (1.02 ∼ 2.51 g/kg DM) and ammonia nitrogen (86.3 ∼ 107 g/kg total nitrogen). All the groups were steady during 14 days of aerobic exposure, while SD group was more stable with lower (p < 0.05) yeast (4.60 vs. 5.17 ∼ 5.77 log10 cfu/g FW) and mould (3.33 vs. 4.12 ∼ 4.64 log10 cfu/g FW) populations than other treated groups on day 14. Moreover, SD group had the highest (p < 0.05) in vitro digestibility of dry matter (67.8 vs. 56.6 ∼ 63.5%) and neutral detergent fibre (61.7 vs. 50.5 ∼ 57.4%) among all groups. Overall, SD is recommended as additive to improve fermentation quality, in vitro ruminal digestibility, and aerobic stability of TMR silage.HIGHLIGHTS After 14 days of aerobic exposure, the yeast and mould populations in TMR silage can be efficiently inhibited by sodium diacetate treatment. Sodium diacetate treatment evidently enhanced in vitro digestibility of dry matter and neutral detergent fibre compared to other treatments. Sodium diacetate is recommended as additive to improve fermentation quality, in vitro ruminal digestibility, and aerobic stability of TMR silage.http://dx.doi.org/10.1080/1828051X.2023.2206422aerobic stabilitychemical additivesfermentation qualitylactic acid bacteria inoculantstotal mixed ration silage |
| spellingShingle | Siran Wang Guanjun Zhang Jie Zhao Zhihao Dong Junfeng Li Tao Shao Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives Italian Journal of Animal Science aerobic stability chemical additives fermentation quality lactic acid bacteria inoculants total mixed ration silage |
| title | Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives |
| title_full | Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives |
| title_fullStr | Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives |
| title_full_unstemmed | Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives |
| title_short | Fermentation quality, aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives |
| title_sort | fermentation quality aerobic stability and in vitro gas production kinetics and digestibility in total mixed ration silage treated with lactic acid bacteria inoculants and antimicrobial additives |
| topic | aerobic stability chemical additives fermentation quality lactic acid bacteria inoculants total mixed ration silage |
| url | http://dx.doi.org/10.1080/1828051X.2023.2206422 |
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