Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration
We newly designed super-elastic biodegradable scaffolds with longitudinally oriented microchannels for repair and regeneration of peripheral nerve defects. Four-armed poly(ε-caprolactone-co-D,L-lactide)s (P(CL-co-DLLA)s) were synthesized by ring-opening copolymerization of CL and DLLA from terminal...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2012-01-01
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| Series: | Science and Technology of Advanced Materials |
| Online Access: | http://dx.doi.org/10.1088/1468-6996/13/6/064207 |
| _version_ | 1819027256010342400 |
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| author | Koichiro Uto, Takanari Muroya, Michio Okamoto, Hiroyuki Tanaka, Tsuyoshi Murase, Mitsuhiro Ebara and Takao Aoyagi |
| author_facet | Koichiro Uto, Takanari Muroya, Michio Okamoto, Hiroyuki Tanaka, Tsuyoshi Murase, Mitsuhiro Ebara and Takao Aoyagi |
| author_sort | Koichiro Uto, Takanari Muroya, Michio Okamoto, Hiroyuki Tanaka, Tsuyoshi Murase, Mitsuhiro Ebara and Takao Aoyagi |
| collection | DOAJ |
| description | We newly designed super-elastic biodegradable scaffolds with longitudinally oriented microchannels for repair and regeneration of peripheral nerve defects. Four-armed poly(ε-caprolactone-co-D,L-lactide)s (P(CL-co-DLLA)s) were synthesized by ring-opening copolymerization of CL and DLLA from terminal hydroxyl groups of pentaerythritol, and acryloyl chloride was then reacted with the ends of the chains. The end-functionalized P(CL-co-DLLA) was crosslinked in a cylindrical mold in the presence of longitudinally oriented silica fibers as the templates, which were later dissolved by hydrofluoric acid. The elastic moduli of the crosslinked P(CL-co-DLLA)s were controlled between 10−1 and 102 MPa at 37 °C, depending on the composition. The scaffolds could be elongated to 700% of their original size without fracture or damage ('super-elasticity'). Scanning electron microscopy images revealed that well-defined and highly aligned multiple channels consistent with the mold design were produced in the scaffolds. Owing to their elastic nature, the microchannels in the scaffolds did not collapse when they were bent to 90°. To evaluate the effect of the channel diameter on Schwann cell migration, microchannels were also fabricated in transparent poly(dimethylsiloxane), allowing observation of cell migration. The migration speed increased with channel size, but the Young's modulus of the scaffold decreased as the channel diameter increased. These findings may serve as the basis for designing tissue-engineering scaffolds for nerve regeneration and investigating the effects of the geometrical and dimensional properties on axonal outgrowth. |
| first_indexed | 2024-12-21T05:39:34Z |
| format | Article |
| id | doaj.art-3a2801fd10e849f2bbb06c53db143172 |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-12-21T05:39:34Z |
| publishDate | 2012-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-3a2801fd10e849f2bbb06c53db1431722022-12-21T19:14:18ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142012-01-01136064207Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migrationKoichiro Uto, Takanari Muroya, Michio Okamoto, Hiroyuki Tanaka, Tsuyoshi Murase, Mitsuhiro Ebara and Takao AoyagiWe newly designed super-elastic biodegradable scaffolds with longitudinally oriented microchannels for repair and regeneration of peripheral nerve defects. Four-armed poly(ε-caprolactone-co-D,L-lactide)s (P(CL-co-DLLA)s) were synthesized by ring-opening copolymerization of CL and DLLA from terminal hydroxyl groups of pentaerythritol, and acryloyl chloride was then reacted with the ends of the chains. The end-functionalized P(CL-co-DLLA) was crosslinked in a cylindrical mold in the presence of longitudinally oriented silica fibers as the templates, which were later dissolved by hydrofluoric acid. The elastic moduli of the crosslinked P(CL-co-DLLA)s were controlled between 10−1 and 102 MPa at 37 °C, depending on the composition. The scaffolds could be elongated to 700% of their original size without fracture or damage ('super-elasticity'). Scanning electron microscopy images revealed that well-defined and highly aligned multiple channels consistent with the mold design were produced in the scaffolds. Owing to their elastic nature, the microchannels in the scaffolds did not collapse when they were bent to 90°. To evaluate the effect of the channel diameter on Schwann cell migration, microchannels were also fabricated in transparent poly(dimethylsiloxane), allowing observation of cell migration. The migration speed increased with channel size, but the Young's modulus of the scaffold decreased as the channel diameter increased. These findings may serve as the basis for designing tissue-engineering scaffolds for nerve regeneration and investigating the effects of the geometrical and dimensional properties on axonal outgrowth.http://dx.doi.org/10.1088/1468-6996/13/6/064207 |
| spellingShingle | Koichiro Uto, Takanari Muroya, Michio Okamoto, Hiroyuki Tanaka, Tsuyoshi Murase, Mitsuhiro Ebara and Takao Aoyagi Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration Science and Technology of Advanced Materials |
| title | Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration |
| title_full | Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration |
| title_fullStr | Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration |
| title_full_unstemmed | Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration |
| title_short | Design of super-elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for Schwann cell migration |
| title_sort | design of super elastic biodegradable scaffolds with longitudinally oriented microchannels and optimization of the channel size for schwann cell migration |
| url | http://dx.doi.org/10.1088/1468-6996/13/6/064207 |
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