Protocol for electrical modulation of ERK signaling dynamics in cell lines

Protocol for electrical modulation of ERK signaling dynamics in cell lines

Summary: Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity...

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Bibliographic Details
Main Authors: Kan Zhu, Liang Guo, Preena Patel, John Albeck, Quan Qing, Wolfgang Losert, Min Zhao
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Cell culture
Microscopy
Signal transduction
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006323

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http://www.sciencedirect.com/science/article/pii/S2666166722006323

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