Toward a genome scale sequence specific dynamic model of cell-free protein synthesis in Escherichia coli

In this study, we developed a dynamic mathematical model of E. coli cell-free protein synthesis (CFPS). Model parameters were estimated from a dataset consisting of glucose, organic acids, energy species, amino acids, and protein product, chloramphenicol acetyltransferase (CAT) measurements. The mod...

Full description

Bibliographic Details
Main Authors: Nicholas Horvath, Michael Vilkhovoy, Joseph A. Wayman, Kara Calhoun, James Swartz, Jeffrey D. Varner
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Metabolic Engineering Communications
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2214030118300452