Combined 15N-Labeling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis

Combined 15N-Labeling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis

Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the dynamics of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification of substra...

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Bibliographic Details
Main Authors: Nicola V. Huck, Franz Leissing, Petra Majovsky, Matthias Buntru, Christina Aretz, Mirkko Flecken, Jörg P. J. Müller, Simon Vogel, Stefan Schillberg, Wolfgang Hoehenwarter, Uwe Conrath, Gerold J. M. Beckers
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-12-01
Series:Frontiers in Plant Science
Subjects:
mitogen-activated protein kinases
Arabidopsis thaliana
plant immunity and development
MKK7 cascade
tandemMOAC
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.02050/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2017.02050/full

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