Efficient and markerless gene integration with SlugCas9-HF in Kluyveromyces marxianus
Abstract The nonconventional yeast Kluyveromyces marxianus has potential for industrial production, but the lack of advanced synthetic biology tools for precise engineering hinders its rapid development. Here, we introduce a CRISPR-Cas9-mediated multilocus integration method for assembling multiple...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-07-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-06487-w |