The Current State-of-the-Art Identification of Unknown Proteins Using Mass Spectrometry Exemplified on De Novo Sequencing of a Venom Protease from <i>Bothrops moojeni</i>

The Current State-of-the-Art Identification of Unknown Proteins Using Mass Spectrometry Exemplified on De Novo Sequencing of a Venom Protease from <i>Bothrops moojeni</i>

(1) Background: The amino acid sequence elucidation of peptides from the gas phase fragmentation mass spectra, de novo sequencing, is a valuable method for the identification of unknown proteins complementary to Edman sequencing. It is increasingly used in shot-gun mass spectrometry (MS)-based prote...

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Bibliographic Details
Main Authors: Simone König, Wolfgang M. J. Obermann, Johannes A. Eble
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Molecules
Subjects:
snake venom
gas phase peptide ion fragmentation
mass spectrometry
spectrum quality
MS/MS
CID
Online Access:https://www.mdpi.com/1420-3049/27/15/4976

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https://www.mdpi.com/1420-3049/27/15/4976

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