Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells

Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells

Summary: This protocol presents the use of SARS-CoV-2 isolates to infect human kidney organoids, enabling exploration of the impact of SARS-CoV-2 infection in a human multicellular in vitro system. We detail steps to generate kidney organoids from human pluripotent stem cells (hPSCs) and emulate a d...

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Bibliographic Details
Main Authors: Elena Garreta, Daniel Moya-Rull, Megan L. Stanifer, Vanessa Monteil, Patricia Prado, Andrés Marco, Carolina Tarantino, Maria Gallo, Gustav Jonsson, Astrid Hagelkruys, Ali Mirazimi, Steeve Boulant, Josef M. Penninger, Nuria Montserrat
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell culture
Microbiology
Microscopy
Stem Cells
Cell Differentiation
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007523

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http://www.sciencedirect.com/science/article/pii/S2666166722007523

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