Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites
A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-12-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/11/1/40 |
| _version_ | 1811187063030022144 |
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| author | Staffan Kjelleberg Torsten Thomas Francesco Ballestriero Malak Daim Anahit Penesyan Suhelen Egan |
| author_facet | Staffan Kjelleberg Torsten Thomas Francesco Ballestriero Malak Daim Anahit Penesyan Suhelen Egan |
| author_sort | Staffan Kjelleberg |
| collection | DOAJ |
| description | A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source. |
| first_indexed | 2024-04-11T13:55:45Z |
| format | Article |
| id | doaj.art-3ae7afc70e8349559260e6836f31899c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:55:45Z |
| publishDate | 2012-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3ae7afc70e8349559260e6836f31899c2022-12-22T04:20:20ZengMDPI AGMarine Drugs1660-33972012-12-01111404910.3390/md11010040Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive MetabolitesStaffan KjellebergTorsten ThomasFrancesco BallestrieroMalak DaimAnahit PenesyanSuhelen EganA common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source.http://www.mdpi.com/1660-3397/11/1/40antibacterialantinematodefunctional genomic screenfosmid librariesmarine bacteriamarine bioactivesCaenorhabditis elegans |
| spellingShingle | Staffan Kjelleberg Torsten Thomas Francesco Ballestriero Malak Daim Anahit Penesyan Suhelen Egan Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites Marine Drugs antibacterial antinematode functional genomic screen fosmid libraries marine bacteria marine bioactives Caenorhabditis elegans |
| title | Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites |
| title_full | Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites |
| title_fullStr | Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites |
| title_full_unstemmed | Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites |
| title_short | Assessing the Effectiveness of Functional Genetic Screens for the Identification of Bioactive Metabolites |
| title_sort | assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| topic | antibacterial antinematode functional genomic screen fosmid libraries marine bacteria marine bioactives Caenorhabditis elegans |
| url | http://www.mdpi.com/1660-3397/11/1/40 |
| work_keys_str_mv | AT staffankjelleberg assessingtheeffectivenessoffunctionalgeneticscreensfortheidentificationofbioactivemetabolites AT torstenthomas assessingtheeffectivenessoffunctionalgeneticscreensfortheidentificationofbioactivemetabolites AT francescoballestriero assessingtheeffectivenessoffunctionalgeneticscreensfortheidentificationofbioactivemetabolites AT malakdaim assessingtheeffectivenessoffunctionalgeneticscreensfortheidentificationofbioactivemetabolites AT anahitpenesyan assessingtheeffectivenessoffunctionalgeneticscreensfortheidentificationofbioactivemetabolites AT suhelenegan assessingtheeffectivenessoffunctionalgeneticscreensfortheidentificationofbioactivemetabolites |