Protein Engineering of a Pyridoxal-5′-Phosphate-Dependent <span style="font-variant: small-caps">l</span>-Aspartate-α-Decarboxylase from <i>Tribolium castaneum</i> for β-Alanine Production

Protein Engineering of a Pyridoxal-5′-Phosphate-Dependent <span style="font-variant: small-caps">l</span>-Aspartate-α-Decarboxylase from <i>Tribolium castaneum</i> for β-Alanine Production

In the present study, a pyridoxal-5&#8242;-phosphate (PLP)-dependent L-aspartate-&#945;-decarboxylase from <i>Tribolium castaneum</i> (TcPanD) was selected for protein engineering to efficiently produce &#946;-alanine. A mutant <i>Tc</i>PanD-R98H/K305S with a 2.45...

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Autors principals: Xin-Jun Yu, Chang-Yi Huang, Xiao-Dan Xu, Hong Chen, Miao-Jie Liang, Zhe-Xian Xu, Hui-Xia Xu, Zhao Wang
Format: Article
Idioma:English
Publicat: MDPI AG 2020-03-01
Col·lecció:Molecules
Matèries:
β-alanine
pyridoxal-5′-phosphate (plp)
<span style="font-variant: small-caps">l</span>-aspartate-α-decarboxylase
directed evolution
characterization
whole-cell bioconversion
Accés en línia:https://www.mdpi.com/1420-3049/25/6/1280

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https://www.mdpi.com/1420-3049/25/6/1280

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