Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry

Examination of Various Metal Ion Sources for Reducing Nonspecific Zinc finger−Zn2+ Complex Formation in ESI Mass Spectrometry

The formation of zinc finger peptide−Zn2+ complexes in electrospray ionization mass spectrometry (ESI-MS) was examined using three different metal ion sources: ZnCl2, Zn(CH3COO)2, and Zn(OOC(CHOH)2COO). For the four zinc finger peptides (Sp1-1, Sp1-3, CF2II-4, and CF2II-6) that bind only a single Zn...

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Bibliographic Details
Main Authors: Soojin Park, Sunhee Park, Joo Yeon Oh, Sang Yun Han, Kyubong Jo, Han Bin Oh
Format: Article
Language:English
Published: Korean Society for Mass Spectrometry 2012-09-01
Series:Mass Spectrometry Letters
Subjects:
Zinc finger
Zinc ion
Electrospray ionization
Tartrate
Noncovalent complexes
Online Access:http://www.msletters.org/journal.do?method=currentIssue&journalSeq=J000011&menuId=0202&introMenuId=0201

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http://www.msletters.org/journal.do?method=currentIssue&journalSeq=J000011&menuId=0202&introMenuId=0201

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