Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng
A metabolomics approach was used to profile metabolites of Panax notoginseng fermented with Aspergillus cristatus in two ways, liquid-state fermentation (LF-P) and solid-state fermentation (SSF-P) and examine metabolite markers representing antioxidant activity and skin anti-aging. Protopanaxadiol (...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.602135/full |
| _version_ | 1818279838086070272 |
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| author | Sunmin Lee Sunmin Lee Chagam Koteswara Reddy Jeoung Jin Ryu Seoyeon Kyung Yonghwan Lim Myeong Sam Park Seunghyun Kang Choong Hwan Lee |
| author_facet | Sunmin Lee Sunmin Lee Chagam Koteswara Reddy Jeoung Jin Ryu Seoyeon Kyung Yonghwan Lim Myeong Sam Park Seunghyun Kang Choong Hwan Lee |
| author_sort | Sunmin Lee |
| collection | DOAJ |
| description | A metabolomics approach was used to profile metabolites of Panax notoginseng fermented with Aspergillus cristatus in two ways, liquid-state fermentation (LF-P) and solid-state fermentation (SSF-P) and examine metabolite markers representing antioxidant activity and skin anti-aging. Protopanaxadiol (PPD) and protopanaxatriol (PPT) contents were higher in SSF-P than in LF-P and showed a multiplicative increase over the fermentation period of four days. PPD and PPT levels also correlated with antioxidant and anti-aging effects in skin, based on the mRNA expression of dermal extracellular matrix components. In the bioactivity validation assays, PPD and PPT significantly improved the expression of type-I collagen, fibrillin-1, and elastin in human dermal fibroblasts from both young and old subjects; these were comparable with the effects of the SSF-P extracts. Overall, our results suggest that changes in the metabolites of P. notoginseng fermented with A. cristatus enhance the quality and availability of bioactive compounds associated with skin anti-aging. |
| first_indexed | 2024-12-12T23:39:41Z |
| format | Article |
| id | doaj.art-3afba4cbd3b94ee78d31fd858a538201 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T23:39:41Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3afba4cbd3b94ee78d31fd858a5382012022-12-22T00:07:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-12-011210.3389/fmicb.2021.602135602135Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginsengSunmin Lee0Sunmin Lee1Chagam Koteswara Reddy2Jeoung Jin Ryu3Seoyeon Kyung4Yonghwan Lim5Myeong Sam Park6Seunghyun Kang7Choong Hwan Lee8Department of Bioscience and Biotechnology, Konkuk University, Seoul, Seoul, South KoreaResource Analysis Research Laboratory, Korea Ginseng Corporation, Daejeon, South KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul, Seoul, South KoreaCOSMAX BTI R&I Center, Pangyo inno valley E, 255 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South KoreaCOSMAX BTI R&I Center, Pangyo inno valley E, 255 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South KoreaCOSMAX BTI R&I Center, Pangyo inno valley E, 255 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South KoreaCOSMAX BTI R&I Center, Pangyo inno valley E, 255 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South KoreaCOSMAX BTI R&I Center, Pangyo inno valley E, 255 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, South KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul, Seoul, South KoreaA metabolomics approach was used to profile metabolites of Panax notoginseng fermented with Aspergillus cristatus in two ways, liquid-state fermentation (LF-P) and solid-state fermentation (SSF-P) and examine metabolite markers representing antioxidant activity and skin anti-aging. Protopanaxadiol (PPD) and protopanaxatriol (PPT) contents were higher in SSF-P than in LF-P and showed a multiplicative increase over the fermentation period of four days. PPD and PPT levels also correlated with antioxidant and anti-aging effects in skin, based on the mRNA expression of dermal extracellular matrix components. In the bioactivity validation assays, PPD and PPT significantly improved the expression of type-I collagen, fibrillin-1, and elastin in human dermal fibroblasts from both young and old subjects; these were comparable with the effects of the SSF-P extracts. Overall, our results suggest that changes in the metabolites of P. notoginseng fermented with A. cristatus enhance the quality and availability of bioactive compounds associated with skin anti-aging.https://www.frontiersin.org/articles/10.3389/fmicb.2021.602135/fullPanax notoginsengAspergillus cristatussolid-state fermentationmass spectrometryprotopanaxadiolprotopanaxatriol |
| spellingShingle | Sunmin Lee Sunmin Lee Chagam Koteswara Reddy Jeoung Jin Ryu Seoyeon Kyung Yonghwan Lim Myeong Sam Park Seunghyun Kang Choong Hwan Lee Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng Frontiers in Microbiology Panax notoginseng Aspergillus cristatus solid-state fermentation mass spectrometry protopanaxadiol protopanaxatriol |
| title | Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng |
| title_full | Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng |
| title_fullStr | Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng |
| title_full_unstemmed | Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng |
| title_short | Solid-State Fermentation With Aspergillus cristatus Enhances the Protopanaxadiol- and Protopanaxatriol-Associated Skin Anti-aging Activity of Panax notoginseng |
| title_sort | solid state fermentation with aspergillus cristatus enhances the protopanaxadiol and protopanaxatriol associated skin anti aging activity of panax notoginseng |
| topic | Panax notoginseng Aspergillus cristatus solid-state fermentation mass spectrometry protopanaxadiol protopanaxatriol |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.602135/full |
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