A Soluble Fluorescent Binding Assay Reveals PIP2 Antagonism of TREK-1 Channels

A Soluble Fluorescent Binding Assay Reveals PIP2 Antagonism of TREK-1 Channels

Lipid regulation of ion channels by low-abundance signaling lipids phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid (PA) has emerged as a central cellular mechanism for controlling ion channels and the excitability of nerves. A lack of robust assays suitable for facile detection of...

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Bibliographic Details
Main Authors: Cerrone Cabanos, Miao Wang, Xianlin Han, Scott B. Hansen
Format: Article
Language:English
Published: Elsevier 2017-08-01
Series:Cell Reports
Subjects:
PIP2
TREK
TRAAK
Kir
potassium channel
fluorescent assay
lipid binding
arachidonic acid
signaling lipid
phosphatidic acid
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717310008

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http://www.sciencedirect.com/science/article/pii/S2211124717310008

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