Effects of error, chimera, bias, and GC content on the accuracy of amplicon sequencing

Effects of error, chimera, bias, and GC content on the accuracy of amplicon sequencing

ABSTRACTTargeted amplicon sequencing is widely used in microbial ecology studies. However, sequencing artifacts and amplification biases are of great concern. To identify sources of these artifacts, a systematic analysis was performed using mock communities comprised of 16S rRNA genes from 33 bacter...

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Bibliographic Details
Main Authors: Yujia Qin, Liyou Wu, Qiuting Zhang, Chongqin Wen, Joy D. Van Nostrand, Daliang Ning, Lutgarde Raskin, Ameet Pinto, Jizhong Zhou
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:mSystems
Subjects:
targeted gene amplicon sequencing
MiSeq
chimeric sequence
sequence error
sequence GC content
bias
Online Access:https://journals.asm.org/doi/10.1128/msystems.01025-23

Internet

https://journals.asm.org/doi/10.1128/msystems.01025-23

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