Optimization of The Cell Aggregates Method for Isolation and Purification of Human Granulosa Cells from Follicular Fluid

Background Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGCs) and other contaminating cell types. Several strategies, such as the antibody binding methods, the flask method, the cell strainer and positive selection of granulosa aggregates after density gradient (DG) ce...

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Bibliographic Details
Main Authors: Georges Raad, Marwa Bazzi, Judy Tanios, Youmna Mourad, Joan Azouri, Joseph Azouri, Chadi Fakih
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2020-01-01
Series:International Journal of Fertility and Sterility
Subjects:
Online Access:http://www.ijfs.ir/article_45568_7b25708df568ab1e32f0a0526661071f.pdf