Optimization of The Cell Aggregates Method for Isolation and Purification of Human Granulosa Cells from Follicular Fluid
Background Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGCs) and other contaminating cell types. Several strategies, such as the antibody binding methods, the flask method, the cell strainer and positive selection of granulosa aggregates after density gradient (DG) ce...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Royan Institute (ACECR), Tehran
2020-01-01
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Series: | International Journal of Fertility and Sterility |
Subjects: | |
Online Access: | http://www.ijfs.ir/article_45568_7b25708df568ab1e32f0a0526661071f.pdf |